The Study of Association between Apolipoprotein C3 Gene Polymorphism and Hypertensive Disorder Complicating Pregnancy
|Keywords||Hypertensive disorders in pregnancy Apolipoprotein C3 Gene polymorphism|
Hypertensive disorder complicating pregnancy (HDCP) is acharacteristic disease during pregnancy period. It is one of thediseases that seriously threatened maternal and perinatal safety andis still one of the main causes of maternal and perinatal mortality sofar. There were many pathogenetic hypotheses about hypertensivedisorder complicating pregnancy, but it is not clear yet. so it is veryimportant to study the etiology in order to prevent and cure HDCP.Literatures reported that lipid metabolic disorder wasassociation with HDCP. Genetic factor was proofed contributing tothe risk of HDCP. And the SstI polymorphism of the apo C3 gene isassociated with HTG in many races. Considered these three reasonwe designed this study to reveal association betweenapolipoprotein C3 gene polymorphism and hypertensive disordercomplicating pregnancy. Methods: In our study, 84 patients with HDCP and 81 normalcontrols were recruited. They were all single birth, primiparity andmatched for age. DNA was extracted from peripheral blood.Genotyping was performed by using PCR-based restrictionfragment length polymorphism (RFLP) method. The products ofenzyme digestion were separated by electrophoresis in 2.5%agarose gel, dyed with EB and analysed by gel aerial image system.Datum were analysed with SPSS software.Results: 1.The genotypic distribution was in agreement withHardy-Weinberg equilibrium。2.The frequencies of Apo C3 SstⅠS1S1, S2S1, S2S2 genotypes were 48.81%, 36.90% and 14.29%respectively in HDCP group, and in control group were 54.32%,35.80% and 9.87% respectively. Between control group and HDCPgroup the frequencies distribution of genotypes was no statisticallysignificant difference ( χ 2=0.9075 P>0.05). 3.The frequencies ofthe two alleles(S1、S2)were 72.22% and 27.78% respectively inHDCP group, and were 67.06% and 32.94% respectively in controlgroup.Prevalence of S2 allele in normal controls and HDCPpatients were comparable(P>0.05). 3. There was no statisticaldifference in genotypic distribution between HDCP1 complicgroup and HDCP2 complic group. And there was no statisticallysignificant in allele frequencies comparing gestational hypertensionsubset, mild-pre-eclampsia subset, severe-pre-eclampsia subset,and eclampsia subset. 4.There was no statistical difference in thesystolic pressure of different Apo C3 SstⅠgenotypes(P>0.05), aswell as in the diastolic pressure (P>0.05). The difference in thepositive rate of proteinuria of the different Apo C3 SstⅠgenotypeswas no statistically significant(P>0.05). 5.The results of relativerisk analysis of Apo C3 SstⅠgenotypes distribution and alleleswere,S2S2/S1S1:OR=1.6098(95%CI:0.5960～4.3484),S2S2/S2S1:OR=1.4032(95%CI:0.5013～3.9280),S2S1/S1S1:OR=1.1842(95%CI:0.5362～2.6155),S2+/S2-:OR=1.2472(95%CI:0.9674～1.6080). Genotypes S2S2 and S2S1 didn’tincrease the risk of HDCP in our study, or S2 allele did neither.Conclusion: Our study didn’t show a significant associationbetween Apo C3 gene SstⅠ polymorphism and HDCP. And ApoC3 gene SstⅠ polymorphism wasn’t associated with the severityof HDCP either in our study. Genotypes S2S2 and S2S1 didn’tincrease the risk of HDCP in our study, or S2 allele did neither.We failed to show an association between the S2 allele of apoC-III and HDCP. The lack of association between HDCP and apoC-III SstⅠ polymorphism may be a result of the limited number ofpatients included in our study, or the fact that we studid only onepolymorphism of apo C-III gene, which had more than 5polymorphsim as we knew. Future larger works will need to clarifywhether the apo C-III polymorphism affects susceptibility toHDCP.