Dissertation > Medicine, health > Surgery > Traumatology > Trauma

Effect of Microcurrent Stimulation on the Keratinocyte Proliferation in Vitro

Author YueHaiLing
Tutor PengDaiZhi
School Third Military Medical University
Course Surgery
Keywords microcurrent keratinocyte HaCaT cell proliferation culture model
CLC R641
Type Master's thesis
Year 2005
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Background: It was confirmed that electrostimulation has an accelerating effect on wound healing in vivo. Both cell migration and proliferation of skin epidermis were two major cases during the reepithelization process of wound healing. In a few years there are some researches about cell migration in the electrical fields, but the reports about cell proliferation influenced directly by electrical current are hardly found. Electrical current is frequently served as parameter in the electrical stimulation therapeusis. To elucidate the mechanisms of the electrostimulatory effect on accelerating wound healing, it is necessary to set up a culture model of cells proliferation stimulated by electrical current. And then, the observations on cell proliferation stimulated by electrical current are all based on this instituted model. The understanding of the mechnisms of the electrostimulatory effect would benefit the development of more effective protocol for clinical electrostimulation therapeusis.Objectives: (1) To institute a culture model of cells proliferation stimulated by electrical current. (2) To observe effects of electrical current on cell proliferation, the relation between the maximal effect and the culture time,and the dose effect by the different amperages.Methods: (1) To execute the microcurrent power apparatus according to requirement of the experiment. (2) To make two culture chambers with platinum thread electrode or the nickel-chromium alloy electrode, and compare the effects of microcurrent on these two kinds of electrodes. (3) The proliferation of keratinocytes primary was evaluated by propidium iodide staining and flow cytometer analysis. And then the culture model of keratinocytes stimulated by microcurrent was set up. (4) The HaCaT cells were cultured in this model and divided into the control group without electrostimulation and electrical stimulation group which was stimulated by microcurrent (10 μA, 5 min /once, twice/day, 5 days). The OD value of these two groups was measured by MTT method at different day. The net OD value between electrical stimulation group and control group was calculated. The days of culture with the maximum net OD value was selected. At the selected

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