Dissertation
Dissertation > Medicine, health > Preventive Medicine,Health > Epidemiology and Prevention of Disease > Epidemiology of the basic theory and methods > Special epidemiological each

Molecular Epidemiology Study on the Hereditary and Environmental Affecting Factors of hyperlipidemia

Author ShuiLiMing
Tutor ChenKun
School Zhejiang University
Course Epidemiology and Biostatistics,
Keywords hyperlipidemia Heredity Environment Affecting factor Molecular Epidemiology
CLC R181.3
Type Master's thesis
Year 2002
Downloads 279
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As the important risk factor of Atherosclerosis, coronary heart disease, stroke and hypertension, hyperlipidemia is a popular disease which severely hurt the health of masses. Satisfactory primary and secondary prophylactic effect result will be achieved if prevention measures are adopted early. The happening and development of hyperlipidemia is comprehensive affecting result of genetic and environmental factors, while gene polymorphism of apolipoprotein, lipoprotein receptor and lipoprotein metabolism enzyme are the material base of heredity, the living custom of parents also posses a fairly effect.In this study, prevalence survey and case-control study were used to investigate the main environmental factors which affect the happening of hyperlipidemia in island inhabitant and the heritability of the disease; Modem biological experimental technique was used to detect the gene polymorphism of apoB 3’ allele. The expected studying result will be used as scientific proofs to prevent or reduce the happening of hyperlipidemia.Materials and MethodsThe study field was in Mayi island, Putuo county, Zhoushan city. 40 pedigrees were selected randomly from 395 pedigrees, 271 persons in the selected pedigreeswere than defmited as studying object. According to the diagnosis criteria, the objects were diagnosed as hyperlipidemia cases, the other objects were than defmited as controls.The content of epidemiological questionnaire included personal condition, eating habit , smoking history, wine drinking history, disease history and menstrual history. The questionnaires were filled in the field by investigator after asking questions to the studying object face to face.Diet intake of each person was investigated through the assessment of food intake quantity and frequency using food model. The yearly nutrients intake of each person were calculated using Matrix calculate .10ml vein blood was collected from elbow vein on an empty stomach, which was than used to detect the level of lipoprotein and apolipoprotein and DNA extraction. The gene polymorphism of apoB 3’VNTR was also detected after PCR amplication. The detecting items of lipoprotein included TC, TG, HDL-C, LP(a), while the items of apolipoprotein included ApoA 1, ApoA2, ApoB, ApoC2, ApoC3, ApoE.Univariable and local multivariable Logistic regression analysis, 2 independent samples of nonparametric tests were used to compare the difference of varied variables between cases and controls, variables which P level were less than 0.2 were selected into multivariable Logistic regression to identify the main affecting factors of hyperlipidemia. Heritability was calculated using falconer method. Path analysis was used to assess the comprehensive effect of inheritance and environment.Result572 objects were identified as hyperlipidemia from 265 objects who had been detected the level of lipoprotein, the total prevalence of object was 27.17%, while theprevalence of men and women was 23.97% and 29.86% respectively. The prevalence of hypercholesterolemia, hypertriglyceridemia and combined" hyperlipidemia was 36.11%, 45.83%, 18.06% respectively. There were no significant difference in prevalence and disease structure between men and women. The prevalence of hyperlipidemia in men and women showed a tendency of increase with age. The level of TG, TC, LDL-C increased significantly after menopause in women.The result of univariable analysis show that illiteracy and married were the risk factors of hyperlipidemia, advanced analysis show that it’s a false correlation which caused by a confounding factor-age. There was no relationship between eating habit and hyperlipidemia. Regular dinner and intake fruit more than 10 times a moon were protection factors of hyperlipidemia.Smoking was a important risk factor of hyperlipidemia in men, there was significant dose-effect relationship between hyperlipidemia prevalence and smoking index. The high quantity of yearly alcohol intake(more than 15000ml)and the early drink-beginning age(early than 20) were risk fa

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