Dissertation > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors

Experimental Study on Targeting Therapy for Human Primary Hepatocellular Cancer by Fusion Gene

Author ShengQinSong
Tutor ZhangJie
School Kunming Medical College
Course Surgery
Keywords human primary liver cancer target fusion gene gene therapy angiostatin gene human simplex herpes virus II thymidine kinase gene
CLC R735.7
Type Master's thesis
Year 2006
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Objective To study the target treating effective of nude mouse model transplanted with human liver cancer cell strain SMMC-7721 by pcDNA3/AFP/TK/Angio fusion gene. Method To cultivate human liver cancer cell strain SMMC-7721 and construct nude mouse sub-skin model transplanted with human primary liver cancer by 5×106-0.2ml-1. When the diameter of the tumor got to 4~5mm, the nude mice were divided randomly into five groups (6 mice per group): control group ; vector group ;GCV group ; pcDNA3/ TK/Angio group ;and pcDNA3/AFP/TK/Angio group. Different plasmids were directly injected into tumor and GCV intraperitoneal injection was given simultaneously according to different groups. Mice were killed on the 2th day after the 2th intratumoral injection and the 15 day after the 3 intratumoral injection. Observing the growth condition of tumor; pathologic examination; detecting AFP in serum of nude mouse by radiation immunization; detecting the MVD and VEGF expression by immunohistochemistry. Results 1. The twice results of tumor growth condition, pathologic examination, AFP, MVD and VEGF expression indicated that pcDNA3/TK/Angio group and pcDNA3/AFP/TK/Angio group could remarkably inhibit the growth of tumor and were more effective than control group; vector group; and GCV group(P<0.05). 2. The 2th result of AFP, MVD and VEGF

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