Xinjiang the Qiemo ancient crowd mtDNA polymorphisms research
|School||Tianjin Normal University|
|Keywords||Ancient DNA Mitochondrial DNA Pedigree analysis Genetic structure Qiemo|
The development of contemporary molecular genetics has opened up many new areas of social and human science research . Application and Development of PCR technology in the 1980s , so the study of the DNA of the remains and relics of ancient creatures into possible ancient DNA research emerging multidisciplinary field of research . Xinjiang Qiemo Jiawa Ai date grams cemetery of the ancient population of the Spring and Autumn Period matrilineal genetic structure made ??more systematic study . Individual teeth from 25 cases were successfully extracted mitochondrial DNA (mtDNA) , and the use of the direct sequencing method hypervariable region (HVR-Ⅰ and HVR-Ⅱ) sequence information , combined with innovative use of PCR-RFLP alleles specific PCR (AS-PCR) technology to obtain the coding region haplotype -specific single nucleotide polymorphisms (SNPs) sites . Comparative analysis of mtDNA sequences collected 13,103 cases of the modern world population reference samples studied haplogroups vesting accurately divided . Matrilineal genetic structure of the Qiemo ancient populations were analyzed on the basis of credible data . The results show : the Qiemo ancient gene pool of the population that exists Eurasian western type (n = 15,60%), there are also the the Eurasian eastern type ( n = 10,40 % ) . By comparison with the the modern Xinjiang region population , found that the the ancient the Qiemo population of genetic component may be genetic composition of modern Minority contribute . Combined with the results of the study of archeology and anthropology , we speculated that this ancient population live in an age or earlier period , Europe and Asia between the eastern and western populations may have occurred gene exchange . We have demonstrated that the genetic structure of the Xinjiang the Qiemo ancient crowd is more complex than we expected , which reflect the complexity of the history of Xinjiang and Central Asia . But there is no doubt that the ancient DNA research is to uncover the mystery of human history is very useful and essential tool . Ancient DNA techniques to study the ancient population of the Xinjiang region help to elucidate the history of Eurasia populations of migratory exchanges .