Security Evaluation on Dietary Soybean Meal Replacement by Cottonseed Meal of Cyprinus Carpio
|School||Huazhong Agricultural University|
|Keywords||Cyprinus carpio cottonseed meal replacement soybean meal toxicity|
The purpose of this dissertation was to investigate the effect of substitution of soybean diet by cottonseed diet on growth performance, muscle flavor and quality, histological structure of liver and kidney, reproductive performance of the male and some other related physiological and biochemical parameters in Cyprinus carpio. The control group of fish was fed the basal ration of soybean meal in combination with fish meal that did not contain any cottonseed. In three experimental groups of A, B and C, soybean protein were isonitrogenously replaced by cottonseed protein at 50%, 75% and 100% respectively. Fish, which initially weighted 4.23±0.36g were fed for 42 days to assay WGR, SGR and FCR, and were fed 120 day to assay the body chemical composition, flavor amino acid, essential amino acid, total amino acid and tissue lesion of liver and kidney. Fish, weighted 197.07±9.14g, were reared for 56 day for biochemical analysis. The function of liver and kidney, GPT, GOT, Urea, PT, PC, K~+, TP, Glu, and TG in serum under the same condition and diets above were assayed. The main results were as follows.The results showed that 50% supplementation of cottonseed in the ration did not exhibit any adverse effects on growth performance, muscle quality and flavor, and the structure of liver and kidney of fish weighted 4.23±0.36g, and also on liver and kidney function and development, fertility performance, and the content of K~+, TP, Glu and TG of fish weighted 197.07±9.14g.Given the ration with 75% cottonseed, there was no significant effect on growth performance, muscle quality and flavor, and the structure of liver and kidney of fish weighted 4.23±0.36g, and also on the liver development, kidney function and development, fertility performance, and the content of K~+, TP, Glu and TG of fish averaging 197.07±9.14g. However the liver function of fish weighted 197.07±9.14g was inhibited significantly at 56d. As a result it was toxic to fish weighted 197.07±9.14g with a long time feeding.Given the ration with 100% cottonseed, the muscle quality, flavor and structure of kidney of fish weighted 4.23±0.36g were not influenced, but the growth performance declined significantly and liver lesion was observed. Although the liver development, kidney function and development, fertility performance, and the content of K~+ of fish weighted 197.07±9.14g did not differ significantly from control group (P>0.05), the liver function declined greatly 42d later and the content of TP, Glu and TG reduced clearly at 56d (P<0.05). So it was not safe to fish weighted 4.23±0.36g and 197.07±9.14g. Therefore, it was not suggested to apply in aquaculture with this diet.