Dissertation > Industrial Technology > Chemical Industry > Basic Organic Chemistry Industry > Production of natural organic compounds

Studies on the Application of Making Astaxanthin from Natural Lutein by Modification

Author DongXuLi
Tutor ZhangLiXin
School Qingdao University
Course Applied Chemistry
Keywords Lutein Astaxanthin Hydrolysis reaction Isomerization reaction Oxidation reaction
Type Master's thesis
Year 2006
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The research process into astaxanthin , lutein ester as raw material . The process comprises lutein ester hydrolysis, isomerization of lutein and zeaxanthin , bromination, oxidation . Study lutein ester hydrolysis , purification of the best production process route : hydrolysis of lutein esters and alcohols alkali solution at 56 ℃ . Complete hydrolysis after washing with warm water , centrifuged, and freeze-dried to obtain high purity lutein crystals . By ultraviolet - visible spectrophotometry and high performance liquid chromatography for qualitative and quantitative detection , the purity of the product reaches 70 % , yield 60% . Lutein ester hydrolysis kinetic model . The results show that , in the case of large excess of alkali , can be approximately regarded as a reaction of the reaction . 56 ℃ , 100g lutein ester was dissolved in 90ml of ethanol , hydrolyzed 20g under the action of a base , the Hydrolysate free lutein in the expression of the relationship between the concentration and the reaction time is : C Lutein = 0.435 [ 1- exp ( - 0.02305t ) ] where : t is the reaction time , min; Hydrolysate C lutein for the time t of the concentration of free lutein , mol / L . Experimental study how by chemical reaction, the lutein crystals Production isomerizes isomers - zeaxanthin , and by the separation and purification to obtain a high content of zeaxanthin . Lutein crystals with an alcohol in an alkali solution and emulsifier, under high temperature reaction in an inert environment 20h. By HPLC analysis, the reaction conversion rate reached 70% or more . After the reaction product was purified to obtain a high purity zeaxanthin monomer . HPLC method for qualitative and quantitative detection, the zeaxanthin content of 3% from the initial to the final 50% . Experimental Study of a brominated the zeaxanthin monomer from high purity , oxidation of astaxanthin production process . The zeaxanthin mixing with the oxidant solution , adding a halogenating agent . The inert environment reaction of 4 ~ 6h. Reaction and then washed with water purification . High performance liquid chromatography and mass spectrometry for qualitative and quantitative detection . Reactions ultimately generate astaxanthin content of 10% .

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