Studies on the Application of Making Astaxanthin from Natural Lutein by Modification
|Keywords||Lutein Astaxanthin Hydrolysis reaction Isomerization reaction Oxidation reaction|
The research process into astaxanthin , lutein ester as raw material . The process comprises lutein ester hydrolysis, isomerization of lutein and zeaxanthin , bromination, oxidation . Study lutein ester hydrolysis , purification of the best production process route : hydrolysis of lutein esters and alcohols alkali solution at 56 ℃ . Complete hydrolysis after washing with warm water , centrifuged, and freeze-dried to obtain high purity lutein crystals . By ultraviolet - visible spectrophotometry and high performance liquid chromatography for qualitative and quantitative detection , the purity of the product reaches 70 % , yield 60% . Lutein ester hydrolysis kinetic model . The results show that , in the case of large excess of alkali , can be approximately regarded as a reaction of the reaction . 56 ℃ , 100g lutein ester was dissolved in 90ml of ethanol , hydrolyzed 20g under the action of a base , the Hydrolysate free lutein in the expression of the relationship between the concentration and the reaction time is : C Lutein sub > = 0.435 [ 1- exp ( - 0.02305t ) ] where : t is the reaction time , min; Hydrolysate C lutein sub > for the time t of the concentration of free lutein , mol / L . Experimental study how by chemical reaction, the lutein crystals Production isomerizes isomers - zeaxanthin , and by the separation and purification to obtain a high content of zeaxanthin . Lutein crystals with an alcohol in an alkali solution and emulsifier, under high temperature reaction in an inert environment 20h. By HPLC analysis, the reaction conversion rate reached 70% or more . After the reaction product was purified to obtain a high purity zeaxanthin monomer . HPLC method for qualitative and quantitative detection, the zeaxanthin content of 3% from the initial to the final 50% . Experimental Study of a brominated the zeaxanthin monomer from high purity , oxidation of astaxanthin production process . The zeaxanthin mixing with the oxidant solution , adding a halogenating agent . The inert environment reaction of 4 ~ 6h. Reaction and then washed with water purification . High performance liquid chromatography and mass spectrometry for qualitative and quantitative detection . Reactions ultimately generate astaxanthin content of 10% .