Of catgut embedding of morphine withdrawal rats erythrocyte immune function
|School||Guiyang College of Traditional Chinese Medicine|
|Course||Acupuncture and Massage|
|Keywords||Zusanli Guan Yuan Catgut embedding Morphine withdrawal Erythrocyte immune Experimental study|
Purpose: opioid withdrawal through a variety of ways to reduce the body's immune function, non-specific immunity, cellular and humoral immune function is severely impaired. The experimental contemplated by Zusanli Guanyuan catgut embedding method to observe the morphine withdrawal rats RBC immune adhesion function, to investigate the of catgut embedding of RBC immune function and provide experimental basis for clinical use. Methods: 40 Wistar rats were randomly divided into four groups, each group of 10, respectively, for the normal group, model group, scopolamine treatment group (referred to as the drug group), catgut implantation group (referred to as the the temple the Buried group), injected subcutaneously The morphine hydrochloride establish acute morphine addiction model by naloxone reminder addiction, withdrawal symptoms score based on the score sheet. The Kuok method for the determination of red blood cell C3b receptor rosette rate (RBC-C3bRR), red blood cell immune complex rosette (RBC-ICR). The using electronic hemocytometer determination of the the RBC total number of use erythrocyte C3b receptor to promote rate and the suppression rate detection RFER, RFIR activity. Results: (1) modeling the end 3h reminder addiction model group, drug group and hole buried group withdrawal score increased, normal group differences were very significant significance (P lt; 0.01), modeling success. 2h reminder addiction, model group, drug group and hole buried group after the end of treatment withdrawal total score has decreased, the end of the modeling 3h reminder addiction than, the differences were statistically significant (P lt; 0.01), and the normal group differences were very significant with sexual significance (P lt; 0.01), drugs and cave buried with the model group differences, respectively, have a significant meaning and very significantly with sexual significance (P lt; 0.05 lt; 0.01), but acupoint buried the line is better than drugs (P lt; 0.05); (2) the weight of the rats before the model was no difference in the modeling 3h, the end of the model group, drug group and catgut implantation group rats significant weight loss than with the normal group, the differences were very significant meaning (P lt; 0.01), again urging addiction 2h after the end of treatment found, model group, drug group and the catgut embedding rats weight than when the end of the modeling 3h restored normal group than differences there are very significant with sexual meaning and differences there are significant with sexual significance (P lt; 0.01, lt; 0.05), drugs and the acupoint catgut embedding with the model group differences there are very significant with sexual significance (P lt; 0.01), but drugs and cave buried treatment recovery effect on body weight equivalent between the two was not statistically significant. (3) the total number of model rats erythrocyte than decreased significantly with normal group, the difference was very significant (P lt; 0.01); compared with the model group, the the erythrocyte total number of drug group with catgut implantation group was significantly higher (P lt ; 0.01), but the drug group catgut embedding group showed no differences (P gt; 0.05) (4) model group rat red blood cell C3b receptor rosette reduce elevated red blood cell immune complex rosette rate, compared with normal group the differences were very significant meaning (P lt; 0.01) C3b receptor was significantly elevated after drugs with catgut embedding therapy, red blood cell immune complex rosette rate decreased with the model group, the differences were very significant (P lt; 0.01), but the drug group with catgut implantation group showed no differences (P gt; 0.05); (5) model group Erythrocyte Immune promoting factor reducing immunosuppressive factor increased, compared with normal group differences There are very significant (P lt; 0.01), drugs and catgut embedding therapy to boost the immune promoting factor increased, suppression of the immune inhibitory factor increased, with the model group, the difference was significant (P lt; 0.01) However, no statistically significant difference between the drug and the catgut embedding (P gt; 0.05). Conclusion: Zusanli the Guanyuan catgut embedding improve withdrawal symptoms and weight by enhancing Mor withdrawal rats erythrocyte immune adhesion function to promote the body Rehabilitation.