Dissertation > Medicine, health > Oncology > Oral cavity, maxillofacial tumors

Elemene Inhibits the Expression of eIF4E and VEGF in Human SACC-83 Cell

Author LiuHong
Tutor ChenKuiSheng;WenHongTao
School Zhengzhou University
Course Pathology and Pathophysiology
Keywords Elemene Human adenoid cystic carcinoma cell eIF4E VEGF Immunocytochemistry
CLC R739.8
Type Master's thesis
Year 2007
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Human adenoid cystic carcinoma is one of the common oral malignancy, serious harm to human life and health, harm to human body lies not only in the tumor cells grow out of control caused by clonal dysplasia, at the same time, a powerful tumor growth, invasion and metastatic potential of salivary adenoid cystic carcinoma of the major causes of death and tumor angiogenesis is a prerequisite for malignant tumor growth and metastasis. Tumor through tumor blood vessels from the host to get rich nutrition and output depend on it to host a large number of malignant cells to cause tumor growth and metastasis. Tumor angiogenesis in a variety of growth factor stimulation, such as epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), transforming growth factor (TGF), platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF), etc.. Wherein, VEGF secreted by certain tumor cells and can induce tumor angiogenesis, to maintain the continued growth of the tumor and binds to receptors on the vascular endothelium, is an important angiogenic factor, related to a number of physiological and pathological processes. Experiments show abnormally high expression of VEGF in most solid tumors, is the most specificity of angiogenic growth factor one. Eukaryotic translation initiation factor of eIF (eukaryotic initiations for factor eIF) family members (of eIF4E, eIF4G, etc.) start hat dependent protein translation process. Cells under normal physiological conditions of eIF family members low expression, when the eIF family members overexpression causes cell growth acceleration, and morphological change, and even lead to carcinogenesis. Wherein eIF4E overexpression can help the choice of the body upstream of translation initiation sites, causing the increase in the translation of VEGF mRNA. VEGF overexpression caused changes in tissue phenotype, tumor cells, development, invasion and metastasis. Elemene (elemene) is our first extracted from the rhizomes of Zingiberaceae warm turmeric (Curcuma) anticancer active ingredient. The study found that elemene on human salivary adenoid cystic carcinoma SACC-83 cell line, the inhibition was dose-dependent. To investigate the inhibitory effect of elemene on human salivary adenoid cystic carcinoma SACC-83 cells explore elemene on human salivary adenoid cystic carcinoma cell line eukaryotic translation initiation factor eIF4E and tumorigenesis The blood vessels of the vascular endothelial growth factor (VEGF) protein expression. This study immunocytochemistry detection of human salivary adenoid cystic carcinoma SACC-83 cells eIF4E expression of VEGF protein levels change to explore down of eIF4E, VEGF expression and activity of inhibition of human adenoid cystic carcinoma invasion, clinical elemene and cisplatin combination therapy as soon as possible to provide some theoretical basis used in adenoid cystic carcinoma clinical. Materials and Methods adenoid cystic carcinoma SACC-83 cells provided by the the Oral Hospital biology Institute. After recovery, the incubation passage is divided into 10 experimental groups. Salivary adenoid cystic carcinoma SACC-83 cells were treated with different concentrations of elemene respectively, adenoid cystic carcinoma SACC-83 cells were treated with cisplatin plus elemene SACC-83 cells was observed at different times morphology, Histochemical immune cells were detected by SP eIF4E protein expression, the same method of detection and tumor angiogenesis and VEGF expression. 2 using SPSS 11.0 software for statistical analysis. Measurement data are expressed as mean ± standard deviation ((?) ± s) using analysis of variance of the experimental data were statistically analyzed test α = 0.05 for the test of significance level. Results 1 concentration elemene down SACC-83 cells eIF4E and VEGF protein expression compared to twenty-two elemene with the control group, the differences were statistically significant (P <0.05). The elemene cut of eIF4E, VEGF protein expression in a time and dose dependent, with the the elemene role time extension, eIF4E, VEGF protein expression in turn decline. (60μg/ml) and cisplatin (3μg/ml), 3 elemene combination can strengthen SACC-83 cell growth inhibition. Conclusion 1 SACC-83 cells eIF4E and VEGF protein highly expressed on tumor angiogenesis growth factor. Lam Hong ene cisplatin inhibit SACC-83 cell growth, has a time-and concentration-dependent. Elemene, have a higher rate of growth inhibition of cisplatin combination, the two drugs used in combination may further improve the suppression of the drugs on the growth of tumor cells. Immunocytochemistry detected of eIF4E and expression of the growth factor VEGF in tumor angiogenesis, found the elemene role group of eIF4E, VEGF expression than the blank and solvent control group; prompted elemene inhibit the growth of not only protein synthesis suppression, but also with the eIF family members decreased expression of cause-related protein (VEGF).

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