Dissertation > Medicine, health > Ophthalmology > Lens and vitreous diseases > Cataract

The Functional Study of Congenital Cataract-causing Gene Heat Shock Factor 4(HSF4)

Author LiDuanZhuo
Tutor RenXiang
School Huazhong University of Science and Technology
Course Genetics
Keywords Cataract Human lens epithelial cells Heat shock factor 4 Confocal Microscopy
CLC R776.1
Type Master's thesis
Year 2009
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HSF4 belongs to a family of heat shock factor (HSF) HSF in cells subjected to pressure to survive will activate its target genes . Mutations can cause autosomal dominant and recessive congenital cataracts . HSF4b protein is the HSF4 lens in the main shear this . So far , on HSF4b protein mutation causes congenital cataracts mechanism is unclear . The laboratory HLE ( human lens epithelial cells ) cells HSF4b protein cDNA . I will restructure into pEGFP-N1 vector transfected HLE cells using confocal microscopy revealed HSF4b spotty aggregation in the nucleus . Its positioning manner similar to the spliceosome , and reported in the SC35 spliceosome can interact with certain transcription factor , we assume that the transcription factor HSF4b same as also may interact with . But will HSF4b SC35 connected to the different fluorescently labeled vector and co-transfected HLE cells and found that they are not co-localization . Then , I select the other two sub- nucleus structure labeled molecules - coilin and gamma -H2AX , they are card Hou body and DNA damage repair sites labeled molecules immunofluorescence experiments confirmed them with HSF4b is not colocalization . For HSF4b this new positioning , we will try other domains of Nuclear test with which they colocalization if they can co-localization , we can take advantage of this research known Nuclear domains through it to found HSF4b functionality . If you prove colocalization HSF4b not with all the Nuclear domains , indicating that it represents a new Nuclear domains , we will try other methods of finding a protein or other molecule HSF4b to do with each other , to understand this new Nuclear domains as well as its function in the cell and lens .

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