The Experimental Study of 125I Seeds Interstitial Brachytherapy Combined with External Beam Radiotherapy for Lewis Lung Cancer in C57bl/6 Mice
|School||Nanjing Medical University|
|Keywords||Lung tumors Radiotherapy Iodine radioisotopes Mice|
125 sup> I particle inter-organizational experimental mice with Lewis lung implantation combined with external beam radiotherapy Objective: To study 125 < / sup > implantation Plus Radiation treatment of lung cancer , the effectiveness of the I particle inter-organizational . Methods: C57BL / 6 mice Lewis lung carcinoma (LLC) solid tumor model , divided into three groups , according to a completely random method , simply 125 sup> implanted between the I particle organization radiotherapy group , simple external radiotherapy group ( 15Gy ) 125 sup> implantation combined with external radiotherapy ( 125 sup> I outside the radiotherapy 8Gy ) between the I particle organization group ( n = 6 ) mice . Packet processing 3d tumor volume was measured 15 days later , the mice were sacrificed and measured tumor weight inhibitory rate was calculated , the production of tumor tissue samples , routine pathological examination , immunohistochemical method detecting tumor microvessel density (MVD) expression . Single-factor analysis of variance differences between groups , line SNK q test . Results: Compared with the control group , simple radiotherapy group , the group of simple external radiotherapy (15Gy) and combined with radiotherapy group the ( LLC volume have a different degree of growth inhibition ( q 125 sup> the I particle radiotherapy 8Gy ) = 11.06,17.13,16.31, P lt ; 0.05) , simple external radiotherapy group and the combination group most obvious ( inhibition rates were 50.9% , 48.4% ) , although both tumor weight difference was not statistically significant ( q = 0.50, P gt; 0.05), but combined with radiotherapy tumor inhibition rate is higher than the simple radiotherapy group ( 48.4% versus 28.6% ) . Immunohistochemical results showed that 4 mice tumor MVD values ??were (23.33 ± 4.84 ) , ( 17.50 ± 3.67) , ( 11.83 ± 2.14 ) (12.67 ± 3.39 ) microvessels / high power field ( × 200 ) , pure within radiotherapy MVD values ??group than the control group , and higher than the combined radiotherapy group , the difference was statistically significant (q = 3.92 and 3.25 , all P lt ; 0.05 ), no statistically significant differences between the radiotherapy group in the combination group alone (q = 0.57, P gt; 0.05). Conclusion: Radiotherapy can inhibit tumor growth by reducing tumor MVD, reducing angiogenesis . Achieve the same rate of local tumor control , the United 125 sup> absorbed dose to reduce the I particle inter-organizational implantation irradiation can be effective in reducing the required dose of external beam radiation to the surrounding normal tissues and organs .