Investigation on Relation between the Espression of VEGF-C、VEGFR-3 and Podoplanin and Lymphatic Metastasis in Human Hepatocellular Carcinoma
|School||Guilin Medical College,|
|Keywords||Hepatocellular carcinoma Vascular endothelial growth factor C Vascular endothelial growth factor receptor 3 podoplanin Lymphatic Lymphatic microvessel density|
Purpose of hepatocellular carcinoma (hepatocellular carcinoma, HCC) is one of the most common malignant tumor worldwide, ranking third in cancer deaths in China. Lymph node metastasis is an important determinant of the clinical prognosis of hepatocellular carcinoma (HCC), but on hepatocellular carcinoma control the lymphangiogenesis mechanisms poorly understood. Confirmed in various animal models of vascular endothelial growth factor-C (vascular endothelial growth factor C, VEGF-C) promotes tumor lymphangiogenesis and tumor cells in lymph node metastasis and distant metastasis. Podoplanin is a relative molecular mass of 38 × 103 type I transmembrane glycoprotein, which is a specific marker of lymphatic endothelial cells. This article explores the vascular endothelial growth factor C and vascular endothelial growth factor receptor 3 (vascular endothelial growth factor receptor, VEGFR-3) expression and its role in the progression of hepatocellular carcinoma hepatocellular carcinoma tissue. Observation of lymphatic endothelial cells podoplanin expression, morphological basis for the study of the molecular mechanisms of hepatocellular carcinoma lymphatic metastasis. Method 1, take our hospital from April 2002 to November 2008 surgical removal of lymph nodes and 56 cases of hepatocellular carcinoma pathology specimens by the pathology of paraffin-embedded tumor and liver. Pathology results will be divided into the lymph node metastasis and lymph node metastasis. 2, the application of the biopsy-use two-step (non-biotin) Immunohistochemical detection reagent used to detect specimens VEGF-C, VEGFR-3 and podoplanin protein expression, detection of cancerous tissue micro-lymphatic vessel density (Lymphatic micro -vessel density, LMVD). Results of VEGF-C and VEGFR-3 expression in hepatocellular carcinoma within the cytoplasm of cancer cells, hepatocellular carcinoma tissue VEGF-C and VEGFR-3 expression was significantly higher than the normal liver tissue (P lt; 0.01). Hepatocellular carcinoma and vascular endothelial cells podoplanin staining, podoplanin positive expression of lymphatic endothelial cells, lymphatic microvessel after counting the number of cancer organizations and cancer adjacent liver tissue LMVD significantly higher than the normal liver tissue (P lt; 0.01). Conclusion 1, VEGF-C expression in hepatocellular carcinoma tissues was significantly higher, presumably generated by the combination of VEGFR-3 promotion lymphatic vessels in hepatocellular carcinoma. 2, Podoplanin optional expression in hepatocellular carcinoma lymphatic endothelium can clear distinction vascular and lymphatic ingredients, can be used as a lymphatic endothelial cell specific markers. 3, LMVD expression in hepatocellular carcinoma tissues was significantly higher, and the increase in the number of lymphatic vessels may be associated with lymph node metastasis of hepatocellular carcinoma.