Study on the Expression and Significance of IL-17 in Primary Liver Cancer
|School||Huazhong University of Science and Technology|
|Keywords||IL-17 liver cancer microvessel density|
PartⅠExpression of IL-17 and microvessel density in Primary liver CancerObjective: To investigate the expression of IL-17 and its association with vascular density in primary liver cancer.Methods: The expression of IL-17 was detected by the RT-PCR. Primary liver cancer tissues were divided into IL-17-positive group and IL-17-negative group. To assay the angiogenesis, the cancer tissues were stained with anti-CD34 antibody by immunohistochemistry.Results: IL-17 mRNA expressed in 26 (70.3%) of the 37 samples of primary liver cancer tested. The microvessel count in IL-17-positive samples (66.6±2.5) was significantly higher than that in IL-17 negative samples (26.7±2.5) of primary liver cancer.Conclusion: The expression of IL-17 could be observed in a considerable proportion of primary liver cancer. The expression of IL-17 was correlated with angiogenesis in primary liver cancer. PartⅡThe effects of exogenous IL-17 in established mouse tumor modelObjective: To explore the impact of IL-17 expression on tumor growth in experimental models of murine liver carcinoma and potential mechanisms.Methods: H22 were used to established experimental models of murine liver cancer. For in vivo assay, H22-bearing mice were injected IL-17 or NS via tail vein, and the tumor volume were detected. To assay the angiogenesis, the tumor tissues from H22-bearing mice with or without injection of IL-17 were stained with anti-CD31 antibody by immunohistochemistry.Results: IL-17, injected into H22-bearing mice, markedly enhanced in vivo tumor growth and significantly increased tumor vascularity.Conclusion: Exogenous IL-17 probably promotes tumor growth through enhancing angiogenesis in vivo.