Dissertation
Dissertation > Medicine, health > Oncology > Gastrointestinal Cancer > Esophageal tumors

Expression of 67LR, MMP-7 and TIMP-1 in Esophageal Squamous Cell Carcinoma and Its Significance

Author WangXiaoLan
Tutor ChenKuiSheng;ZhangHongXin
School Zhengzhou University
Course Pathology and Pathophysiology
Keywords Esophageal squamous cell carcinoma 67LR MMP-7 TIMP-1 Infiltration Shift
CLC R735.1
Type Master's thesis
Year 2009
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Background and Purpose: malignant tumor invasion and metastasis of multiple factors are involved and regulating the complex process, may be involved in tumor cell invasion force, adhesion and stromal interactions. 67 kD laminin receptor (67kDlaminin receptor, 67LR) is a laminin, a non-integrin receptors, is a multifunctional protein, both involved in the assembling process of maturation of the ribosome, but also participate in cell signal transduction, as for a variety of viruses membrane receptors. It can interact and laminin regulate tumor proliferation, adhesion, angiogenesis and accelerate the degradation of the extracellular matrix, promoting tumor invasion and metastasis. 67LR expression was elevated in a variety of tumor tissue. Matrix metalloproteinases (matrix metalloproteinases, MMPs) are a class of zinc-dependent protease family, mainly from the degradation of extracellular matrix (extracellular matrix, ECM) in the body, including interstitial collagenase, interstitial lysin, gelatin, etc. . MMP-7 is a minimum molecular weight of the matrix metalloproteinase family, can activate the other members of the family, and the ECM broad spectrum degradation. It is often expressed in the tumor cells, plays an important role in tumor development and metastasis of with many tumor invasion,. Matrix metalloproteinase inhibitors (tissue inhibitor of metalloproteinases, TIMPs) is a specific inhibitor of MMPs. Strongest in all TIMPs, TIMP-1 role as a natural inhibitor of MMP-7, therewith forming a stable complex, inhibiting its activity, and the ECM from the degradation of the negative regulatory effects, thereby inhibiting tumor cell invasion and metastasis. In addition, TIMP-1 can also be used as a growth factor to promote tumor cell growth and proliferation and metastasis. Esophageal cancer is a malignant cancer of the higher prevalence in the world, especially in East Asian countries such as China and Japan were more common after 5-year survival rate of only 20% to 36%. Invasion and metastasis is the basic biological characteristics of malignant tumors, but also lead to a key factor in the death of patients with esophageal cancer, esophageal carcinoma invasion and metastasis mechanisms has become one of the hotspots of the current study. Joint detection 67LR, MMP-7 and TIMP-1 expression in esophageal squamous cell carcinoma and significance of the research, the literature has not been reported. To further clarify esophageal squamous cell carcinoma invasion and metastasis mechanism and look for the inhibition of esophageal squamous cell carcinoma invasion and metastasis in an effective way, the study by immunohistochemistry SP method, were observed in 56 cases of esophageal squamous cell carcinoma, 26 cases of adjacent atypical hyperplastic tissue and 23 cases of normal esophageal mucosal epithelial tissue 67LR, MMP-7 and TIMP-1 protein expression, explore the combined detection of the three indicators in esophageal squamous cell infiltration, the significance of the transfer process, for the prevention and treatment of esophageal squamous cell carcinoma invasion and metastasis provide a theoretical basis. Method 1. Using immunohistochemical method detected 56 cases of esophageal squamous cell carcinoma, 26 cases of adjacent atypical hyperplasia tissues and 23 cases of normal esophageal mucosal epithelial tissue 67LR, MMP-7 and TIMP-1 protein expression. Statistical analysis: All data were statistically analyzed by SPSS 13.0 software. Between the positive rate compared using χ ~ 2 test (chi-square); relationship between two variables were analyzed using correlation analysis. Significance level alpha = 0.05. 1 normal esophageal epithelial tissues, adjacent the atypical organizations and cancer tissue, the 67LR protein expression in turn increased 13.04% (3/23), 53.85% (14/26) and 67.86% (38 / 56) between the two groups, the difference between the cancer group and normal group was significant (P <0.01), atypical hyperplasia group and normal group difference was significant (P <0.01); MMP-7 protein positive expression rate 34.78% (8/23), 76.92% (20/26) and 73.21% (41/56) between the two groups, the cancer group and normal group difference was significant (P <0.01), atypical hyperplasia difference between the normal group was significant (P <0.01); TIMP-1 protein expression rates were higher, respectively, 0% (0/23), 19.23% (5/26) and 42.86% (24/56) between the two groups, the differences were statistically significant (P <0.05). Invaded the submucosal superficial muscle, deep muscle layer and the outer membrane of esophageal squamous cell carcinoma, the 67LR protein expression, respectively, 66.67% (2/3), 80.00% (12/15), 50.00% ( 10/20) and 77.78% (14/18) between the two groups, the difference was not statistically significant (P> .05); MMP-7 protein expression rates were higher, namely 33.33% (1/3) , 46.67% (7/15), 85.00% (17/20) and 88.89% (16/18) between the two groups, the the submucosal group with deep myometrial group, the adventitia group, superficial muscle group with deep muscle layer group, the outer membrane group, the difference was statistically significant (P <0.05); TIMP-1 protein expression rate decreased 66.67% (2/3), 60.00% (9/15), 55.00% (11 / 20) and 11.11% (2/18), esophageal squamous cell carcinoma invasion of the outer membrane of TIMP-1 protein expression was significantly lower than the submucosal groups, superficial muscle group, deep muscle group, the differences were statistically significant (P <0.05). 3.26 cases with lymph node metastasis and 30 patients without lymph node metastasis in esophageal squamous cell carcinoma, the the 67LR protein expression, respectively, 84.62% (22/26) and 53.33% (16/30) compared the two groups, the difference was statistically significance (P <0.05); MMP-7 protein expression rate was 92.31% (24/26) and 56.67% (17/30), compared the two groups, the difference was statistically significant (P <0.01); TIMP- 1 protein expression rate was 23.08% (6/26) and 60.00% (18/30), compared the two groups, the difference was statistically significant (P <0.01). 4.67LR, MMP-7 and TIMP-1 expression in esophageal squamous cell carcinoma correlation shown by statistical analysis: the 67LR protein expression was positively correlated with MMP-7 and TIMP-1 was negatively correlated (P <0.05) ; MMP-7 in esophageal squamous cell carcinoma, and TIMP-1 (P> 0.05). Conclusion 1.67LR, MMP-7, abnormal expression of TIMP-1 protein may be involved in the development and progression of esophageal squamous cell carcinoma. MMP-7, TIMP-1 protein abnormal expression of esophageal squamous cell infiltration, 67LR abnormal expression nothing to do with the infiltration of esophageal squamous cell carcinoma. 3.67LR, MMP-7, TIMP-1 protein abnormal expression with lymph node metastasis. A 4.67LR the expression of MMP-7 was positively correlated with TIMP-1 was negatively correlated; MMP-7 expression of TIMP-1 was no correlation, indicating that the 67LR, MMP-7, TIMP-1 in the development of esophageal squamous cell carcinoma and may act synergistically in the process of invasion and metastasis.

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