Effect of Tumor-associated Macrophages on Endometrioid Adenocarcinoma
|School||Tianjin Medical University|
|Course||Pathology and Pathophysiology|
|Keywords||Endometrial adenocarcinoma Tumor-associated macrophages Matrix metalloproteinase 2 Microvessel density Angiogenesis Estrogen Receptor Progesterone receptor Immunohistochemistry|
Purpose in recent years, foreign study found that macrophages in tumor tissue, tumor-associated macrophages (tumor-associated macrophages, TAMs), in the occurrence of tumor growth, invasion and metastasis in may play an important role. In this study, endometrial adenocarcinoma to the study, by analyzing the relationship between TAMs with clinicopathological parameters, as well as matrix metalloproteinase-2 (matrix metallo proteinase 2, MMP-2) expression and microvessel density (microvessel density, MVD) The relationship between the mainly explore the TAMs endometrial adenocarcinoma invasion and metastasis and angiogenesis; Preliminary of TAMs endometrial adenocarcinoma and estrogen receptor (estrogen receptor, ER), progesterone receptor ( progesterone receptor, PR) the expression of contact. Methods Tianjin Medical University General Hospital, 2003-2007 line hysterectomy specimens of 67 cases of pathologically diagnosed as endometrioid adenocarcinoma. Two-step detection of CD68 immunohistochemical staining of MMP-2, CD34, ER and PR expression in endometrial adenocarcinoma tissue, CD68 and CD34 markers TAMs and MVD. Tumor tissue is divided into two regions of the tumor and tumor edge statistical markers expression. SPSS15.0 statistical package for analysis, the application of two independent samples t-test and rank sum test, χ2 test and correlation analysis, Spearman rank correlation analysis, the test of significance level α is set at 0.05. Results 1, TAMs scattered distribution of focal or into a piece of the tumor and the tumor edge. Were 65 cases (97.0%) tumors and 61 cases (91.0%) of the edge of the tumor distribution of TAMs number of TAMs in the tumor than the number of the edge of the TAMs, the difference was significant statistical significance (P lt; 0.001) . TAMs number of TAMs and tumor edge myometrial infiltration depth ≥ 1/2 to those myometrial invasion lt; 1/2 or invasion (P = 0.035, P = 0.025); edge TAMs number in partners lymph node metastasis than those without lymph node metastasis (P = 0.001). 2, MMP-2 positive expression in endometrial adenocarcinoma tumor cell cytoplasm. Tumor MMP-2 positive rate was 49.3% (33/67), the edge of MMP-2 positive rate was 58.2% (39/67), the edge of MMP-2 positive rate in the tumor MMP-2 positive rate difference has significant statistically significant (P lt; 0.001). Patients were older, FIGO stage higher, and pelvic lymph node positive rate of MMP-2 in tumor metastasis (P = 0.012, P lt; 0.001, P lt; 0.001); higher pathologic grade The depth of myometrial invasion ≥ 1/2 by edge higher positive rate of MMP-2 (P = 0.007, P = 0.041). Tumor edge, TAMs number of MMP-2 positive than the negative of MMP-2, and the difference was statistically significant (P = 0.014). 3, CD34 positive cells were observed in any region of the tumor stroma, tumor MVD and the edge of the MVD values ??the difference was not statistically significant (P = 0.643). Myometrial invasion ≥ 1/2 by tumor MVD values ??higher (P = 0.048); pathological grade, depth of myometrial invasion ≥ 1/2 'edge higher MVD values ??(P = 0.031, P = 0.014). The number of TAMs in the tumor and MVD was positively correlated (P = 0.003). 4, both the number of tumor or tumor edge, ER-positive and ER-negative by TAMs no significant difference in the number of PR-positive and PR negative TAMs were no statistical differences (P gt; 0.05). Conclusion 1 endometrioid adenocarcinoma myometrial infiltration depth ≥ 1/2, the occurrence of pelvic lymph node metastasis, a larger number of TAMs. TAMs may enhance the invasive ability of the tumor cells, affecting the process of endometrioid adenocarcinoma invasion and metastasis. 2, tumor cells, especially the Edge tumor cells, MMP-2 expression enhanced with endometrial adenocarcinoma invasion force is related to the strength. Tumor margin, TAMs in more number of MMP-2 positive interaction of TAMs and tumor cells may affect tumor cell expression levels of MMP-2. 3, TAMs may be through autocrine and paracrine secretion of several growth factors, including angiogenic factors involved in the regulation of angiogenesis in endometrial adenocarcinoma. 4, the studies have not found that there is a correlation between the number of endometrial adenocarcinoma TAMs with ER, PR expression.