Pregnancy-associated Plasma Protein-a Polymorphism and the Risk of Pre-eclampsia
|School||Nanjing Medical University|
|Course||Obstetrics and Gynaecology|
|Keywords||Pregnancy-associated plasma protein A Preeclampsia Gene polymorphism|
Objective: To investigate pregnancy-associated plasma protein A ( Pregnancy -associated plasma protein -A , PAPP- A ) gene polymorphism with preeclampsia correlation . Methods: Using polymerase chain reaction - single strand conformation polymorphism (Polymerase Chain Reaction and Single-Strand Conformation Polymorphism, PCR-SSCP) 129 examples of preeclampsia patients ( 64 cases of mild preeclampsia , severe preeclampsia pre 65 cases ) and 137 patients admitted to hospital for check-ups at the same giving birth in the first Affiliated Hospital of Nanjing Medical normal pregnancies , PAPP-A gene locus S1224Y ( A / C ) ( dbSNP : rs7020782 ) polymorphism analysis , comparison of genotypes and allele frequency difference between the preeclampsia group and mild preeclampsia , severe preeclampsia group and normal group . Results : AC genotype frequency in the preeclampsia group ( 44.2% ) higher than that of the control group ( 31.4 % ) ( P < 0.05 ) ; the AC genotype frequencies in severe preeclampsia group (75.4 % ) was significantly higher than mild eclampsia the early group (12.5%) (P <0.01); C allele frequency in the severe preeclampsia group (40.8 % ) was significantly higher than the mild preeclampsia group ( 18.7 % ) ( P <0.01 ) . Conclusion : PAPP- A gene locus S1224Y (A / C) AC genotype C allele may be preeclampsia important genetic risk factors related to the severity of the disease .