Dissertation > Medicine, health > Obstetrics and Gynaecology > Obstetrics > Pathological pregnancy ( abnormal pregnancy ) > Complications of pregnancy > Polyendocrine and metabolic diseases

The Research of the Association between Glucose Metabolic Abnormal During Pregnancy and Tumor Necrosis Factor-Alpha Gene Polymorphism

Author ZhouBin
Tutor YuXinYan
School Taishan Medical College
Course Obstetrics and Gynaecology
Keywords Gestational diabetes mellitus (GDM) Tumor necrosis factor -α (TNF-α) Gene polymorphism Polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) Enzyme - linked immunosorbent assay (ELISA)
CLC R714.256
Type Master's thesis
Year 2007
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Objective: In this study, in order to detect TNF-α promoter gene polymorphism and plasma TNF-α content and GDM relationship, using the polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) method. Research TNF-α promoter gene polymorphism and the relationship of abnormal glucose metabolism during pregnancy, while plasma TNF-α content was measured by ELISA. Tried by gene frequency plasma TNF-α levels and insulin resistance index (HOMA-IR) and other indicators to clarify the GDM patients TNF-α promoter gene polymorphism and insulin resistance status. This experiment explored from the perspective of molecular biology and the role of inflammatory cytokines in the pathogenesis of GDM, the theoretical basis for in-depth study of diabetes pathogenesis. MATERIALS AND METHODS: selected from June 2003 to January 2006 in the Central Hospital of Taian obstetrics and gynecology clinic routine prenatal care for pregnant women, a total of 156 cases, including 78 cases of GDM group (including 19 cases GIGT), at the same time select normal, healthy pregnant women of the same age group visits and study the same period, 50g glucose screening 78 cases of GDM tendencies as a control group. Maternal age of the study group and the control group, compared to pregnancies were not statistically different (P gt; 0.05), the data comparable. Enrolled in the experiment are Shandong Ji Han people, except for high blood pressure, heart disease, chronic inflammation, metabolic disease, or the use of an effective weight loss drug. Blood was collected 4ml, genotype wherein 2ml for the PCR-RFLP method detection object of study of TNF-α. 2ml blood determination of plasma TNF-α levels, fasting glucose and fasting insulin. : 1.TNF-α promoter-308G → A gene polymorphism could be detected in the GDM group and the normal control group. GDM group compared with the normal control group, the \38.2%, P lt; 0.05), the difference was statistically significant. 2.GDM group compared with the control group, BMI (24.8 ± 0.8 vs 23.5 ± 0.7, P lt; 0.01), plasma TNF-α levels (5.4 ± 1.4 vs 3.6 ± 1.3, P lt; 0.01), and HOMA-IR ( 5.8 ± 1.4 vs 3.5 ± 1.0, P lt; 0.01) were increased, the difference was statistically significant. GA AA \; 0.01), and HOMA-IR (5.6 ± 1.6 vs 3.8 ± 1.2, P lt; 0.01) were increased, and the difference was statistically significant. 4. Various clinical and biochemical indicators that between any two BMI, TNF-α and HOMA-IR were positively correlated. Conclusion: 1TNF-α promoter gene-308G / A polymorphism in the Han population in Shandong Province. 2.TNF-α promoter -308 \3.TNF-α promoter -308 \

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