Hepatitis B Virus X Protein Upregulate the Expression of CD59 and Crry in Mouse Podocytes
|School||Huazhong University of Science and Technology|
|Keywords||Complement regulatory proteins Podocytes Mice CD59 Crry|
Objective: To investigate the hepatitis B virus ( HBV ) X protein (HBx) protein CD59 and Crry expression on cultured mouse podocytes complement regulation . METHODS: Cultured mouse podocytes divided into PT -PCR hepatitis B virus (HBV) X gene transfection group (Ad-X), blank podocytes (B) and adenovirus empty vector transfected group ( Ad ) and the flow cytometry HBVX gene expression of the CD59 and Crry gene and the protein transfection , MTT assay was used to detect the effects of complement activation ; observation of ERK1 / 2 , of P38MAPK PI - 3K pathway inhibitor on podocyte CD59 and Crry expression the impact . Results: podocyte group (B) compared with blank empty vector adenovirus (Ad) on the mouse podocytes CD59 and Crry gene and protein expression had no significant effect (P = gt ; 0.05 ) , transfection HBVX genome (Ad-X) CD59 protein expression levels significantly higher than the B group ( P lt ; 0.005 ) and Ad group (P lt; 0.005), but also in the level of gene expression was significantly higher than that in B group ( P lt ; 0.05) and Ad group (P lt; 0.05). Ad-X group Crry protein levels significantly higher than the B group ( P lt ; 0.005 ) and Ad group (P lt; 0.005), but also in the level of gene expression was significantly higher than that in B group ( P lt ; 0.05 ) and Ad group (P lt; 0.05). Pathway inhibitor added to the Ad-X group , SB203580 group inhibitors Ad-X group comparison , CD59 protein expression was significantly lower ( P lt; 0.01 ) the Crry protein expression was also significantly reduced ( P lt; 0.01 ) . LY294002 group , U0126 , DMSO group inhibitor Ad-X group to compare the protein expression of CD59 and Crry no significant difference (P = gt ; 0.05) . MTT assay was prompted in each serum dilution point , Ad - X cells lysis inhibition was significantly higher than that in group B and Ad group (P = lt; 0.05 ) , B group and Ad group no significant difference in cell viability . Conclusion : HBx may passage through activation P38MAPK raised in mouse podocytes CD59 and Crry protein and gene expression , inhibition of complement mediated foot cell lysis , which may lead to HBV in podocyte latent infection , can not easily be cleared , thereby HBV-GN role occur .