Dissertation
Dissertation > Medicine, health > Surgery > Urology ( urinary and reproductive system diseases) > Kidney disease

Hepatitis B Virus X Protein Upregulate the Expression of CD59 and Crry in Mouse Podocytes

Author YinXiaoLing
Tutor ZhouJianHua
School Huazhong University of Science and Technology
Course Pediatrics
Keywords Complement regulatory proteins Podocytes Mice CD59 Crry
CLC R692
Type Master's thesis
Year 2010
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Objective: To investigate the hepatitis B virus ( HBV ) X protein (HBx) protein CD59 and Crry expression on cultured mouse podocytes complement regulation . METHODS: Cultured mouse podocytes divided into PT -PCR hepatitis B virus (HBV) X gene transfection group (Ad-X), blank podocytes (B) and adenovirus empty vector transfected group ( Ad ) and the flow cytometry HBVX gene expression of the CD59 and Crry gene and the protein transfection , MTT assay was used to detect the effects of complement activation ; observation of ERK1 / 2 , of P38MAPK PI - 3K pathway inhibitor on podocyte CD59 and Crry expression the impact . Results: podocyte group (B) compared with blank empty vector adenovirus (Ad) on the mouse podocytes CD59 and Crry gene and protein expression had no significant effect (P = gt ; 0.05 ) , transfection HBVX genome (Ad-X) CD59 protein expression levels significantly higher than the B group ( P lt ; 0.005 ) and Ad group (P lt; 0.005), but also in the level of gene expression was significantly higher than that in B group ( P lt ; 0.05) and Ad group (P lt; 0.05). Ad-X group Crry protein levels significantly higher than the B group ( P lt ; 0.005 ) and Ad group (P lt; 0.005), but also in the level of gene expression was significantly higher than that in B group ( P lt ; 0.05 ) and Ad group (P lt; 0.05). Pathway inhibitor added to the Ad-X group , SB203580 group inhibitors Ad-X group comparison , CD59 protein expression was significantly lower ( P lt; 0.01 ) the Crry protein expression was also significantly reduced ( P lt; 0.01 ) . LY294002 group , U0126 , DMSO group inhibitor Ad-X group to compare the protein expression of CD59 and Crry no significant difference (P = gt ; 0.05) . MTT assay was prompted in each serum dilution point , Ad - X cells lysis inhibition was significantly higher than that in group B and Ad group (P = lt; 0.05 ) , B group and Ad group no significant difference in cell viability . Conclusion : HBx may passage through activation P38MAPK raised in mouse podocytes CD59 and Crry protein and gene expression , inhibition of complement mediated foot cell lysis , which may lead to HBV in podocyte latent infection , can not easily be cleared , thereby HBV-GN role occur .

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