Study on Bifidobacterium Longum Multiplication in the Circumstance of Pickle
|School||Southwest Agricultural University|
|Course||Storage and processing of agricultural products|
|Keywords||Bifidobacterium longum Pickle Symbiotic relationship Growth factors|
The Bifidobacterium longum inoculated with Lactobacillus plantarum fermented kimchi proliferation. Study the growth characteristics of the Bifidobacterium longum, and acclimatization was carried out and the acid and salt; symbiotic relationship of Bifidobacterium and Lactobacillus; compare several Bifidobacterium proliferation factor, to determine the seed medium , fermentation liquid formulation, optimum fermentation conditions, and a single fermentation products after the mixing ratio, and changes in viable count of Bifidobacterium longum of the product in different packaging conditions. The results showed that: 1. The strain J-1 has a certain oxygen resistance, acid resistance, generally, Salt Poor grow well under 2% salt concentration, salt concentration of more than 3%, poor growth. 2. After acid-resistant and resistant to oxygen domestication, the Bifidobacterium longum resistance in pH4.2 and facultative grow well under aerobic conditions. Physiological and biochemical characteristics of strains of domesticated around the strain did not mutate. 3. Mixed culture can accelerate the acid-producing Lactobacillus plantarum can promote the growth of Bifidobacterium longum, Bifidobacterium longum Lactobacillus plantarum. Different proportions, a viable count of the two strains in the mixed system of inoculation amount that: Bifidobacterium longum to ensure a certain amount of inoculation (5%) of its in the mixing system is necessary to maintain a high viable amount, increasing plant Lactobacillus inoculation ratio can shorten the fermentation time. 4. Jerusalem artichoke, potato, soybean three extracts the proliferation of Bifidobacterium longum, Jerusalem artichoke extract has better proliferation. 2% of the Jerusalem artichoke extract can be achieved by good Bifidobacterium proliferation. 5. Skim milk 20% tomato juice 2% Jerusalem artichoke extract as seed medium acid production speed faster, the number of viable cells of bifidobacteria. The best formulation of the fermentation liquor: 1.0% salt, 20% of the tomato juice, 1.0% glucose, 2.0% of soy milk, 2% Jerusalem artichoke extract. 6. Mixed fermentation conditions were inoculated with a 2:1 ratio, inoculum size 6%, fermentation temperature 36 ° C, initial pH of 6.8. J-3 Bifidobacterium fermentation optimal reaction conditions for the fermentation temperature was 39 ℃, inoculum size 5%, pH 6.8. Lactobacillus plantarum preferred fermentation conditions of 28 ℃, inoculum size 5%. 7. Single culture, Bifidobacterium longum and Lactobacillus plantarum fermentation time 18 ~ 24h and 16h, respectively. 8. Best mixed fermentation conditions, the fermentation 18h after the maximum length viable bifidobacterium 2.63 × 10 - 7 (7.42), pickles odor; mixing single cultured process, the fermentation broth when the Bifidobacterium longum Lactobacillus plantarum fermentation liquid mixing ratio of 1:2, the number of viable cells, 2.75 × 10 ~~ 7 (logarithmic 7.44) maximum flavor, or. The direct inoculation of a certain proportion of Bifidobacterium longum with the Lactobacillus mixed fermentation with Bifidobacterium and Lactobacillus were single fermentation, and then compared to the mixing process the product flavor difference, but the difference between the number of viable cells of Bifidobacterium obvious . 9. Comparison pickle jars, of ordinary flexible packaging and vacuum packaging different time stored at 4 ℃ Bifidobacterium longum number of viable cells found, vacuum packaging can be a good Bifidobacterium longum to maintain a high number of viable cells.