Dissertation > Mathematical sciences and chemical > Chemistry > Analytical Chemistry > Fundamentals of Analytical Chemistry Theory

Studies about the Interactions between the Analytical Reagents and the Biomacromolecules

Author OuJunHong
Tutor YuYing
School South China Normal University
Course Physical and chemical
Keywords Alizarin Red Acid chrome blue K Phenosafranine DNA Bovine serum albumin Sodium dodecyl sulfate Spectrophotometry Equilibrium dialysis method. Cyclic voltammetry Bare silver electrode
CLC O651
Type Master's thesis
Year 2003
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In this paper, analytical reagent alizarin red, acid chrome blue K as a protein probe phenosafranine DNA probes to study their interactions, and the results are as follows: 1. Alizarin Red (AR) and bovine serum albumin (BSA) in the buffer solution with pH = 2.68 interaction studies have shown that both mainly by electrostatic forces combined, and their combination meets the the Pesavento phase allocation model . According to the relative allocation model, we derive the formula of the distribution ratio D of small organic molecules in protein micro-phase and water phase, and absorbance A, distribution ratio D apparent binding constant K and the thermodynamic parameters ΔG, ΔH, ΔS to discuss the impact of the series of concentrations of the anionic surfactant sodium dodecyl sulfate (SDS) of the system, that the SDS mainly by electrostatic forces and hydrophobic interaction affect the interaction of the AR with BSA. 2. Study under the conditions of acid, alkali, acid chrome blue between K (ACBK) and bovine serum albumin (BSA) combined luminosity nature, whereby explore the different system environments ACBK the mechanism of interaction with the BSA differences. The research shows that the reduced and λ max redshift BSA the ACBK absorbance under conditions of pH = 3.78 and pH = 9.91 under the conditions of BSA the ACBK of λ max blue shift. Spectrophotometry and equilibrium dialysis method consistently showed that the binding reaction in line with the the Pesavento proposed phase assignment model. In addition, through the apparent binding constants K, thermodynamic parameters ΔG, ΔH, ΔS calculations and ACBK pH balanced analysis of the conformation of BSA, think in pH3.78 conditions ACBK combined electrostatic force and BSA at pH = 9.91, both hydrophobic force. 3. Phenosafranine (PS) and DNA in the pH = 7.00 Britton-Robinson buffer solution, capable of forming a non-electrically active supramolecular complexes. System using cyclic voltammetry study found that PS mainly adsorbed on the bare silver electrode surface in the form of cations. Under the experimental conditions, PS has a good peak shape of redox peaks. DNA added little effect, but its redox potential E decreased with increasing DNA concentration, the peak current ip, wherein the oxidation peak the drop value Δipa with DNA concentration within a certain range (0.50-8.00mg / L) a linear relationship. In addition, the article also discussed the mechanism of interaction of the PS and DNA that the two combined electrostatic force.

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