Study on the Relationship between Dynamic Change of Nitrogen in Plant and Protein Accumulation in Seed of Soybean
|School||Northeast Agricultural University|
|Course||Crop Cultivation and Farming System|
|Keywords||Soybean Protein Nitrogen Accumulation|
The test selection northeast soybean producing areas of different genotypes of soybean varieties (lines), high-protein Dongnong 42, in protein Suinong 14, low egg Bai Dongnong 163 material. Potted conditions different genotypes soybean grain protein subunit component accumulation characteristics and nitrogen accumulation operation of the dynamic changes in the relationship, from the molecular level to clarify this physiological process cultivation measures promoting control role and quantization effects, and provide a theoretical basis for the the special quality varieties orientation improved technology implementation. Different genotypes soybean grain protein accumulation dynamic trend differences. Dongnong 163, 14 Suinong protein content in grain formation period (30 to 50 days after flowering) changes slightly, flowering 60 to 67 days this time, declined, and then showed a growth trend; Dongnong42 throughout The protein in the grain formation process showed a trend of continuous growth. Three varieties of different speed in different periods of protein accumulation and grain formation late protein accumulation, the accumulation of strength will determine the level of the mature protein content. Dongnong42 higher accumulation and rapid accumulation rate, the final performance of the highest protein content. Accumulation dynamics consistent grain globulin globulin subunits percentage of both changes in the proportion of the total protein and grain protein content in soybean seed formation process. The glycinin subunits is mainly determined by a variety of genetic factors, without nitrogen fertilizer. But different subunit accumulation and accumulation intensity differences. With the Grain Formation, 11S globulin acidic subunit (acidic), the basic subunit (basic) accumulation higher than 7S globulin subunits, once formed gradually stabilized with longer disappear during Kernel Development, performance a strong genetic stability. Soybean 7S globulin α 'γ subunits to form during the late disappear or decrease in the number of phenomena. Globulin subunits content differences between varieties significant nitrogen fertilizer treatments acidic subunit (acidic), basic subunit (basic), 11S globulin content were highly significant differences globulin total protein content differences reached a significant level. Different genotypes of soybean vegetative organs nitrogen percentage of the distribution amount consistent trend. 0-30 days after flowering, the vegetative organs nitrogen content generally shows a growing trend, and reached its highest point in the early stages of grain formation, and then showed a downward trend of the vegetative organs nitrogen content, in grain maturity bottoming. Vegetative organs of the nitrogen distribution amount, the contribution rate performance of the operation efficiency of nitrogen and transshipment nitrogen on grain: leaf> stem> pod skin. Three soybean varieties vegetative organs nitrogen accumulation was highly significant positive correlation between the peak and the operation amount of grain protein content, was a significant positive correlation with grain yield. Protein content and myosin content, 11S globulin content, alkaline the Subunit (basic) content showed a very significant positive correlation with the content of the acidic subunit (acidic), α subunit content was significantly positively related. Different vegetative organs, nitrogen accumulation peak nitrogen translocation were in for: leaves> stems, husks, skins. Therefore, the accumulation of leaf and stem nitrogen, running in grain nitrogen and protein accumulation plays an important role. The high protein Dongnong 42 vegetative organs Nitrogen Accumulation peak early high, the duration is longer than the other two varieties, nitrogen operation efficiency and protein content was significantly positively correlated. Different nitrogen levels on different genotypes of soybean protein relative, absolute, and globulin content of each subunit component accumulation. With the increase in the amount of nitrogen, high-protein varieties Dongnong42 vegetative organs Nitrogen accumulation peak, the amount of nitrogen operation, operation efficiency, nitrogen harvest index, and the operation of nitrogen on grain contribution strings showed an increasing trend; 11S globulin acid Northeast Agricultural University, a master's degree in agronomy from paper sexual subunit (acidic), 7S globulin, the 11s globulin content as well as the globulin total also showed an increasing trend in the relative and absolute content showed the same trend, resulting in soybean grain protein, and thus to obtain more The high protein content, grain protein yield and grain yield. Nitrogen fertilization varieties of protein and low-protein varieties vegetative organs Nitrogen accumulation peak, has little effect on the amount of nitrogen operation, but nitrogen operation efficiency and nitrogen harvest index and operation of nitrogen on grain contribution rate declined. Protein, low-protein varieties acidic subunit (ac idic) content in conditions of low nitrogen fertilizer the 7S globulin e subunit content increased globulin content and total protein content increased. Basic subunit content (basic) and the 7S globulin day each subunit decreased under conditions of high nitrogen fertilizer, thus globulin and total protein content decreased.