Dissertation > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Basic Veterinary Science > Serological > The immunoassay prevention ( vaccination )

The Dynamic Changes of Immunology in Chickens after Inoculation with HVT Vaccine and SEB and after Infection with vMDV

Author ChenZuo
Tutor WeiPing
School Northeast Agricultural University
Course Preventive Veterinary Medicine
Keywords Immunity Chick Turkey HSV vaccine Staphylococcal enterotoxin B Virulent Marek's disease
CLC S852.52
Type Master's thesis
Year 2003
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Marek's disease is caused by Marek's disease virus the chicken a lymphoid tissue proliferative tumor disease. Although the application of the vaccine for the chicks to provide effective protection, but the disease is still one of the problems of the past 50 years, poultry owners want. Continue to strengthen because the virulence of Marek's disease virus, the vaccine fails phenomenon has occurred frequently. Currently, people come to realize that must be established the main vaccine prevention of genetic disease resistance and Biosafety supplemented by a comprehensive control strategy, in order to effectively control Marek's disease. However, the study of these aspects must be as the theoretical basis for Marek's disease pathogenesis and vaccine immune mechanism. However, the complexity of the mechanism of vaccine, the theory has not yet been fully elucidated. Marek's disease pathogenesis and vaccine immune mechanism still need a lot of basic research. Superantigens the emergence of new ideas for the theoretical study. Staphylococcal enterotoxin B (SEB) is to study more of a super-antigen. At present, has been applied to the treatment of neoplastic diseases in humans. SEB poultry reported less, and therefore provide valuable information about SEB in the Chicken vivo immunological effects clarify, for the prevention of tumor disease caused by Marek's disease virus or other viruses. So, in the field of research will have important theoretical and practical significance. In this study, a preliminary study of the dynamic changes on the the chicks inoculated HVT vaccines and superantigen SEB and infection vMDV after Immunology. 1-day-old chicks were randomly divided into four groups, receiving different treatment: the healthy control group, superantigen SEB vaccination group, the the HVT vaccine group and HVT vaccine superantigen SEB co-inoculated group. 1,8,15,22,29 days of age, peripheral blood ANAE ~~ Determination of T lymphocyte number: 2,9,16,23,30 days old, respectively, spleen and thymus T lymphocyte proliferation reaction, peritoneal macrophages release the amount of nitric oxide and its determination within the acid phosphatase activity. Infection vMDV, 31 days old, were divided into five groups, namely health / challenged group superantigen / challenged group, HVT vaccine / challenged group, superantigen and HVT vaccine co-inoculation / challenged group and healthy controls group. Peripheral Blood ANAE ~ T lymphocyte Determination of the number of 42,60,90 days of age, respectively, for each set of chicks; 43,61,91 days old chicks spleen and thymus T lymphocyte proliferative responses in vitro, intraperitoneal macrophages to release the amount of nitric oxide and acid phosphatase activity within determination. The experimental results show that: SEB in young chickens can increase the number of peripheral blood ANAE ~ T lymphocyte, enhanced thymic and splenic T lymphocyte proliferation, so that the level of cellular immune enhancement; SEB has a similar role in the non-specific immune system performance in macrophage release of nitric oxide increased and enhanced intracellular acid phosphatase activity. When groups of chicks by vMDV attack, SEB and HVT vaccine co-inoculated group-specific and non-specific immune response level than SEB inoculation group and separate HVT vaccine group, SEB HVT / V group the stronger anti vMDV role. At the same time further, SEB and HVT vaccine immune protection synergies, both in MD vaccine protection is of great significance. By SEB A preliminary study of the role of the immune system in Chicken, SEB is expected to be able to for the treatment and prevention of certain poultry diseases.

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