Pathological Study on Thiamphenicol Harming Experimentally in Carp
|School||Sichuan Agricultural University|
|Course||Basic Veterinary Science|
|Keywords||Carp Thiamphenicol Harm Pathology|
660mg/kg, the test 1320mg/Kg 2640mg/Kg 5280mg/Kg, and 10560mg/Kg feed thiamphenicol carp (60 ± 5g) 96 days feeding trial. The pathology of the test and control groups carp, changes in blood and muscle thiamphenicol residues. Throughout the test period, the test group carp showed varying degrees of loss of appetite, weight loss, decreased activity, and increase the as thiamphenicol dose of trend showing the severity of the symptoms; late high-dose group showed nerve symptoms. Compared with the control group, the autopsy shows the experimental group carp head kidney enlargement, the color becomes pale yellow; volume enlargement of the liver, gill brittle texture, color fades, part of the region is pale green, gallbladder extreme expansion of the filling: silk swelling, redness; intestinal mild expansion, a small amount of clear liquid accumulation; spleen congestion, texture, brittle; slightly enlarged kidneys, the color becomes yellow or mottled; histology observed in gill lamellae epithelium edema from floating abnormal increase in the gill rakers surface mucous cells; expansion of the central veins and capillaries of the liver congestion, liver cells at the center of the hepatic lobule coagulation necrosis, including how the amount of lymphocytes, mononuclear cell infiltration, the formation of inflammatory reaction with capillary bile duct dilatation, filling bile; degeneration of pancreatic acinar swelling, dissolution, disappear, formed solubility necrosis, melanin - the macrophages Center hyperplasia and aggregation; meningeal edema, hyperemia, cerebral edema, there multiple solubility necrosis, gliosis; the ovarian follicles dissolved disappear, while a small amount of lymphocytes and mononuclear cell infiltration, egg mostly in Phase II; renal tubular epithelial cell swelling, granular degeneration occurs, and the substrate membrane separation, loss of cytoplasm, leaving only the mesh structure, part of the glomerulus increases, film cells and vascular endothelial cell proliferation, Movement rich nuclear phenomena, the latter can be seen the tubular reproduction; increased volume of the thyroid gland, increase in the number increased gland cavity glial content; mucosal layer of mucus cells increased dramatically; spleen, head kidney, the renal lymphoid tissue reversibility of the red blood cells, decrease in the number of white blood cells and melanocytes - macrophage center network -like endothelial cells and hematopoietic stem cells increase in the number of pathological changes; head intrarenal adrenal chromaffin cell swelling, granular degeneration and vacuolar degeneration occurs. Performance of hematology changes to: the number of red blood cells, white blood cell number and hemoglobin content was significantly reduced (p <0.01), total amino acid content decreases, the control group showed a very significant with (P0.01); serum iron content, beta-N-acetyl amino glucose transferase activity and SOD activity increased pre-and then gradually decreased with the control group, the difference was significant (p <0.01). TEM observation the visible liver, spleen, kidney, and head of renal mitochondrial cristae, was dissolved and disappeared, the matrix becomes empty, vacuolization; expansion of the bile ducts in the liver cells, liver cell nuclei often Chromosome; spleen erythrocyte karyotheca expansion head kidney interstitial neutrophils nuclear chromatin, the perinuclear gap widened; renal tubular epithelial cell nuclei irregular nuclear membrane expansion, widened perinuclear cytoplasm showed vacuolar degeneration, the microvilli swelling, off; side shift. The thiamphenicol content of carp muscle of the experimental group and the control group between the highly significant difference (p <0.01).