1.The Role of STAT3 on the Proliferation and Migration of Human Liver Carcinoma Cell Line under the Stimulation of IL-6 Cytokine 2.The Study of 5-Fu+ Focused Ultrasound Surgery on Curing Ileocecal Tumor in Mouse Model
|School||Nanjing Normal University|
|Keywords||Interleukin- 6 Signal transduction and activator of transcription Adenovirus vector Hepatic carcinoma Migrate Proliferation MTT Wound assay|
Liver cancer is the high incidence of malignant tumors in China, widely distributed, the transfer rate is high, but its molecular mechanism is not clear, and the treatment of liver cancer from the signal transduction pathway has been developed for the emerging field of biological treatment. Cytokines in combination with other cell-specific receptor activation - Department of profits signal transduction molecules to complete the mission of its signal transduction, and therefore constitute a complex network of cytokines, regulate the body's immune and with a variety of diseases the occurrence and prognosis are closely related. Interleukin-6 (IL-6) is a cytokine with broad immunomodulatory effects, mainly mediated by two intracellular signal transduction pathways that cascade Ras-MAPK (mitogen-activated protein kinase) pathway and Jak- STAT (signal transducer and activator of transcription) pathway. Signal transducer and activator of transcription STAT (signal transducer and activator of transcription) family of DNA-binding protein, to play the role of transcriptional regulation, and thus a variety of biological effects mediated tyrosine phosphorylation of signaling pathways coupled. Adenoviral vectors (Adenoviral vector) is the important one of the gene transfer vector. As a eukaryotic gene expression vector, adenovirus vector can be loaded up to 7.5kb exogenous DNA replication efficiency is high, and do not integrate with the host chromosome, and therefore relatively safe. In cancer gene therapy, Ad vectors can be used to carry the tumor suppressor gene to reach the affected area to obtain a better therapeutic effect. Adenoviral vectors of these advantages make it favored the research and clinical trials of gene therapy shows strong vitality. This article select signal transduction and activator of transcription factor STAT3 as a research object, which in the human interleukin-6 (Human Interleukin-6 and hIL-6) stimulation of proliferation and migration of hepatocellular carcinoma cells BEL-7402 role, trying to looking for the treatment of liver cancer at the cellular level. 1 was constructed encoding Stat3 gene and dominant negative mutant (dominant - negative) the Stat3 gene the adenoviral vector Ad1.Stat3 and Ad1.Stat3CYF, they are able to correct expression in hepatoma cells and has activity was confirmed by Western blot. dominant negative STAT3 can inhibit wild-type phosphorylation of STAT3, and in combination with wild-type STAT3, forming inactive dimers, STAT3 large concentration in the nucleus can not enter the nucleus and DNA binding functions. 2 Wound assay and MTT method was used to study the migration and proliferation of cells and found that over-expression of STAT3 inhibit liver cancer cells BEL-7402 migration, expression of dominant negative STAT3 has been restored this inhibition. STAT3 has no effect on the proliferation of BEL-7402 hepatoma cells. 3 IL-6 to promote migration system hepatoma cell BEL-7402, but in the over-expression of IL-6 stimulation of STAT3 was inhibited migration of liver cancer cells BEL-7402, inferred STAT3 is not mediated by IL-6 on liver cancer cells in a master's degree thesis first role, and part of the summary of BEL-7402 migration possible through other signal transduction pathways to mediate balance related to the role of liver cancer cells BEL-7402 the ultimate level of mobility and pathways. IL. Six pairs of BEL-7402 hepatoma cell proliferation.