Molecular Evolution and Phylogeny of Some Species of Oedipodidae Based on Cyt b Gene Sequence
|School||Shaanxi Normal University|
|Keywords||Oedipodidae Mitochondrial DNA Cytochrome b gene Molecular Evolution Phylogenetic|
Oedipodidae insects is Acridoidea in larger groups, widely distributed in all parts of the country to cause serious harm of locusts and grassland pests callus locust class belongs to the spot-winged locust groups in the history of our country. Locusts as important pests of agriculture and animal husbandry has been subject to the attention of biologists, biology workers to seek locust control methods for a long time, has been engaged in the locust biology basic research, including system studies. Oedipodidae systematic study or not deep enough, Molecular Systematics far behind the other Orthoptera. Mitochondrial protein-coding genes cytochrome b (Cytb) is one of the most widely used animal molecular phylogeny molecular markers. In this study, starting from the perspective of molecular biology, Cytb as a molecular marker used to design a pair of Cytb gene specific primers material CTB-CTB-2, were obtained by PCR Oedipodidae four subfamilies 14 kinds on behalf of the individual and leprosy Acrididae one kinds representatives of about 580bp of the individual the Cytb part of sequence. The use of molecular biology software Oedipodidae some species of insects Cytb gene sequences of molecular evolution and phylogenetic analysis. Oedipodidae part of the system of insects by constructing phylogenetic tree of evolution to study the relationship to determine its taxonomic status, to provide basic data for the biological control of locusts. Four common achievements: NJ Law, ME Law, MP method and ML method Oedipodidae part of the insect system evolution to study the relationship are several ways to generate molecular tree topology is roughly the same, only in the there are differences in the attribution of individual species. By the analysis and discussion of the results, the following conclusions: 1. Cytb gene sequences of 14 spot-winged locust mitochondrial analysis, Cytb gene sequence showed strong A T bias (71.3%). Third bit A or T more apparent preference (86.5%). At the same time, the A / T bias between species within the same genus exhibit a certain degree of stability. 2. The conversion of nucleotide substitution in the spot-winged locust Cytb gene was significantly higher than transversions. Transversion occurred mainly to TA, AT, mostly in the third codon. The third codon sites high AT content and amino acid variation of the existence of certain correlation. Codon to A, T the end of high frequency, the third is less G of codon usage reflects the Cytb gene on codon usage bias, consistent with the flies and bees. 3. The Daurian ticks as section-level classification disputes, the results of this study tend to spot-winged locust groups as an independent Division. The red shank Oedaleus and Oedaleus infernalis from Cytb gene molecular evolution and systematics research results that they should be more appropriate as the two kinds. Different scab scab locust subfamily and locust subfamily should be merged into a single subfamily. 4. Oedipodidae insects Division next level of analysis, the following conclusions: Oedipodidae oedpoddae the divided into four branches (subfamily): Greenweave Locust support the beam neck Locust branch, crazy locust branch and fly Snake branch. Locusts, three kinds of cloud spots car locust Oedaleus of genus classified as a branch of the - locusts subfamily: Li grasshopper subfamily crazy locust are the wrinkle knee locust is a different crazy locust subfamily different Li the clam genus should be merged into one group (or subfamily), crazy locust subfamily: beam neck locust is a position as a branch also need the support of a wider range of special: the flowers wins Greenweave bee taxonomic status of comparison, consider as a branch. Division within the main groups of the relationship between: \Crazy the locust, different Li snake subfamily species classification is a bit confusing, crazy maggots, different crazy snake: the shape and counseling species characteristics according to the grasp of the other very fuzzy. Therefore, these three genera! '. J vesting vegetarian more points in the evidence for further discussion. 6. Oedipodidae insects research points the use of the C YTB base is prisoners the nuclear 'bitter acid sequence of each species asked taxonomic relations are relatively clear, most of the same tree topology. Prisoners, Cyt b gene in seed spot-winged locust Section is the order of classification and evolutionary relationships, is a valid mark. However, the analysis of the subfamily level, there may be a certain lack. This study evolutionary characteristics and phylogenetic applications Cytb group [g Oedipodidae insects sub preliminary study for to explore China Oedipodidae insects molecular evolution, to establish the level of the Department of Molecular phylogenetic relationships - to provide brief of evidence in biology, help to establish a correct and perfect Oedipodidae insect classification system.