Separation of Eucommia powder the ultrasonic extraction preparation process and chlorogenic acid in Eucommia leaf
|School||Hunan Normal University|
|Keywords||Eucommia leaf Eucommia powder Chlorogenic acid Ultrasonic wave Extract Separate Analysis|
Eucommia flour ( Eucommia extract powder ) low temperature ultrasonic extraction process of the new method . A more systematic study of the raw materials used , the extraction process, purification methods , the results show that the low temperature ultrasonic extraction of new technology and ultrafiltration new technology , Eucommia powder prepared with the traditional high-temperature frying formulation compared Eucommia fine significantly improve the content of the active ingredients in the powder ( trace elements , amino acids and chlorogenic acid , etc.) , the extraction rate of 56.7% , 35.3% reduction in total sugar content , prepared flour products good stability , gloss , knotless block, and all water-soluble . Using orthogonal effect of the ultrasonic frequency , extraction temperature, extraction time and solid-liquid ratio of factors to obtain the optimal process parameters for : ultrasonic frequency 13kHz, temperature 50 ℃ , time 45min, solid to liquid ratio of 1:8 ; using the natural settling agents purification methods alternative to the traditional alcohol sedimentation process, reducing the consumption of a concentration process the energy consumption and an organic solvent , the organics secondary pollution can be prevented . Eucommia powder extraction process based on the paper of the the Eucommia main medicinal active ingredient chlorogenic acid separation process system . Selection of raw materials extraction process, purification methods were explored . The results showed that : dried before the deciduous Eucommia leaf extract Eucommia chlorogenic acid raw materials as well ; Eucommia chlorogenic acid soluble in ethanol and acetone , solubility in cold water , hot water solubility , according to comprehensive analysis of the selection of water ; chlorogenic acid extraction solvent for heat-sensitive material , the use of ultrasonic low temperature extraction only improve the extraction rate also guarantees effective active ingredient is not destroyed ; ultrafiltration ( separation ) to remove protein macromolecules without organic solvent alcohol Shen phase transition occurs , and chlorogenic acid does not decompose , less losses , and finally using macroporous resin further separation , this process is not only easy to operate , the column material is renewable , and to improve the purity of the product . Extraction after recrystallization content of 74.74% chlorogenic acid products .