Mass Production of Prunus Serrulats Tube-shoots and Some Problems and Countermeasures on Forest Trees Micropropagation in Factory
|School||Quartermaster University of PLA|
|Course||Crop Genetics and Breeding|
|Keywords||Cherry Large-scale Tree tissue culture Axillary bud Rosette Glass Weaken Rejuvenation|
In this study, plant tissue culture technology in organ culture, the ways to explore the large-scale production cherry (Prunus serrulata) plantlets; also involved in the process of large-scale production forest plantlets Part of the problem: the degree of explants young and acquisition season on the establishment of a tissue culture system; Robinia (Robinia) and cherry-scale production of glass and the weakening plantlets (GA caused by excessive growth) phenomenon, and the production of countermeasures. _ In the process of exploring the large-scale production of four varieties of cherry plantlets, we mainly uses the induction of axillary buds (lateral buds) axillary bud induction culture system. Plantlets establishment of primary culture, subculture and rooting medium optimization, cherry plantlets special status - rosette problem solving and subculture cycle, following the generation time and transplanting made a systematic test and axillary bud induction of large-scale production of cherry plantlets feasibility program. The growth state of the four varieties of cherry significant difference in the subculture medium, can be set to medium. But they reflect genetic differences in rooting. After testing, we determined that the best hormone combination of these types of cherry varieties rooting medium range. This large-scale production of many varieties of cherry plantlets the hormone rooting medium matching principle. The cherry tree transplanting easier several cherry perlite: peat soil matrix 1:1 transplant survival rate reached more than 90%. Due to the characteristics of most of the forest itself, outer implant the acquisition season and young can affect the establishment of a tissue culture system. By varying degrees of tender chicken feet maple (Acer palmatum) explants the cuckoo (Rhododendron dauricum) shoot apical bud, the older terminal bud and the buds on the reaction medium observation. We believe that the forest explants should be collected spring new semi-woody explants. Production of Robinia and cherry plantlets, Vitrification, the study found, sucrose and agar glass similar way, by adjusting the medium osmotic potential and the lining quality potential to prevent Vitrification occur and inhibit further deterioration of the glass tube seedlings. The high-intensity light can be suppressed further deterioration of the glass. The sealing membrane sealing manner by inhibition of the most significant glass plantlets. This culture flask microenvironment is caused by the vitrified incentives to improve the culture flask microenvironment inhibited vitrification, seedling growth state is preferably in such an environment. According to the experimental and the discovery of other scholars, to infer the possible ways of inducing Vitrification. Also found that cherry glass transition phenomenon with different varieties and differences, the Concubine Yang the most difficult to vitrification, Higashisakuramachi vitrification serious, the Gang head and Oshima cherry in between. In Robinia tissue culture, GA long-term use to causing plantlets slim weakening, test, PP333 on GA-induced Robinia plantlets weaken the targeted inhibition. At the same time, the appropriate concentration of PP333 can promote the occurrence of axillary buds. In the industrial production of plantlets, PP333 GA similar, the amount is not too high, not long-term use.