The Study on Genetic and Ecological Differentiation of Endangered Species Euonymus Chloranthoides Yang
|School||Southwest China Normal|
|Keywords||Jinyun Euonymus Morphological differentiation Allozyme analysis Genetic Diversity Association analysis Endangered plants|
Jinyun Euonymus endangered as the research object, in the determination of the survival of various groups on the basis of environmental factors, using a variety of quantitative analysis methods from two levels of morphological and allozyme 97 of the Jinyun Euonymus 7 populations 29 individual morphological indicators and six allozyme reflect ecological genetic differentiation and its relationship with the environment. The results show that: ● morphological differentiation of variance analysis showed that, in the study of 29 morphological indicators have 8 personality like the variation reached significantly with the level, six reached a very significant level, between individuals in morphology have a certain degree of differentiation. ● the extent of the differences in the morphological traits studied showed: the vegetative organs variation greater than the variation of the reproductive organs, the measured reproductive organs traits variation did not reach a significant level, morphological differentiation is mainly manifested in the characteristics of the vegetative organs on, but also the vegetative organs affected by the environment. ● At the individual level, the morphological characteristics of the measured data clustering results show that the same population of individuals is not complete and orderly together individuals between different populations often chaotically together some large group of characterization does not appear certain populations of individuals, partial differentiation among populations, which also reflects the genetic heterogeneity among populations of individuals within or morphology due to individuals within the population internal microhabitat differences lead to the population heterogeneity. ● clustering results on morphological data on the population level showed that the two East Spa obvious morphological differentiation between populations and other populations, such differentiation may be related to the geographical isolation between them. ● morphological differentiation and environmental factors gray correlation analysis showed that 29 morphological traits, 26 traits impact factor is the total potassium content. Overall, the largest of several environmental factors impact on morphological traits is full of potassium, soil pH, elevation, moisture absorption water content. Be seen from the soil conditions affect the Jinyun Euonymus growth dominant factor. ● detected six enzyme systems: peroxidase, cytochrome oxidase, superoxide dismutase, esterase, amylase, malic acid dehydrogenase. Enzyme bands were detected in 42 alleles of 15, 12 polymorphic loci, monomorphic loci three polymorphic loci percentage P = 80%, the average per locus allelic genes number Su the dangerous plants Shan cloud Euonymus 'and heart sand side extravagant action to 2 ~ tho' Hill 5 Yang) ecological genetic differentiation of A = 1 .8 average per locus allele effective number of Ae = 1.13. He two .416 average expected heterozygosity, actual heterozygosity H0 = 0.774. Showed high levels of genetic diversity .. the level of genetic differentiation among populations is not high, the Gst 0.206, indicating that most of the genetic diversity within populations from , only 20 of the genetic diversity of the war from the population between the genetic distance between populations are small, Nm = 0 .964; show that populations have a certain intensity of gene flow .. locus heterozygosity and environmental factors gray relational grade that you point heterozygosity major impact factor varies, but overall impact of the main several environmental factors is full of potassium, pH, altitude, this gray and the previous morphological characteristics and environmental factors The association analysis concluded basically the same. Analyzed by enzyme bands and locus heterozygosity view, the genetic diversity of the species is still high, but its population has shown a island-like distribution, which will not be conducive to the tomb populations due to the exchange, will result in Miscellaneous reduction of the degree of genetic diversity decreased. Allozyme cluster analysis of the two-state data show that different populations of individuals often mixed together, show that the higher the individual genetic heterogeneity within populations, the genetic diversity through genetic differentiation coefficient derived mainly exist in This conclusion is consistent within populations. Cluster analysis of gene frequencies and genetic identity shows that a relatively large population 5 other species differentiation. The morphological differentiation results show that the populations of 6,7 differentiation with other populations, but the allozyme analysis showed that genetic differentiation among populations is not large, this thread cloud Euonymus morphology affected by the environment.