Population Genetic Structure of Oxya Chinensis from Northern China and the Relationship among Allozyme Genotypes and Malathion Acute Toxicity
|Keywords||Oxya Population genetic structure Differentiation Acute toxicity of malathion Selective lethal effect Allozyme genotypes|
In recent years, along with farming, environment change, and the global impact of abnormal weather conditions, Oxya occurrence has become more serious. As ecological and geographical environment, living in different parts of the Oxya populations with varying degrees of difference. So Oxya prevention research should be based on the population as a unit, by understanding the genetic structure of different populations, to provide basic data for the prevention and treatment research. Studies have shown that acute lethal effects of malathion and Oxya allozyme genotype correlation exists between, so allozyme genotype may be used as potential drug resistance Oxya monitoring indicators. Oxya is a regional locust species, relatively strong dependence on the water, moderate degree of geographic isolation will significantly restrict gene flow between populations, resulting in the generation of genetic diversity within populations and among populations of different regions of genetic differentiation exists. Genetic structure of different populations differentiation makes pesticide resistance monitoring can not be generalized, but to local conditions, different genetic backgrounds. In this paper, allozyme analysis method of Shanxi and Shaanxi Oxya three genetic structure of natural populations and the degree of differentiation among populations. Virulence malathion experimentally measured for each population LC 50 values ??and the corresponding LC 50 concentrations of malathion were treated populations of these Oxya to deal with the number of individuals of 276-533. Select one of the five polymorphisms strong and stable enzyme staining as a molecular genetic marker loci, the application of allozyme analysis and detection of acute toxicity of malathion treatment Oxya individual survival and death of individual genotypes to study the China Oxya population allozyme genotype and lethal effects of malathion possible between correlation. If such a relationship does exist, you can compare different genotypes Oxya mortality differences between individuals, identify sensitive and resistant genotype markers Allozymic investigate markers in Oxya different geographical populations changes in characteristics of the population and to determine the level of resistance. Three genes that twelve seat (Adk, Ck, α-Gpdh, Hex-1, Hex-2, Idh-1, Idh-2, Ldh, Me, Mdh-1, Mdh-2, Pgi and Pgm) equipotential Enzyme analysis showed that three Oxya populations within populations and genetic diversity among populations exist differences. Only Ck, Idh-1 and Mdh-2 in the three populations of monomorphic loci, the remaining 10 loci in at least one polymorphic population (95% criteria). In Ldh locus alleles show a gradual trend, the frequency of allele α in Shanxi Province on behalf of the county population was 0.631 yangmingbu, Taiyuan, Jinzhong Jinyuan population was 0.720, while in Shaanxi Chang'an County Yangqiao population was 0.852. Percentage of polymorphic loci (P) is relatively high (53.8% ~ 61.5%); average number of alleles per locus (A), on behalf of the county in Shanxi population of 2.1 yangmingbu Taiyuan, Shanxi Jinyuan population of 2.4 Shaanxi Chang'an County Yangqiao population of 2.7; average observed heterozygosity values ??(Ho) yangmingbu population was 0.176, 0.177 Jinyuan populations, populations Yangqiao 0.181. In addition Ldh and Pgi in three populations are in line Oxya Hardy-Weinberg equilibrium, the rest of the vast majority of locus genotype frequencies significantly deviated from Hardy-Weinberg equilibrium, Me, and Pgm two locus genotype frequencies in three populations are highly significant deviation from Hardy-Weinberg equilibrium expectations. Although thirteen loci in nine loci F s (?) values ??were significantly or very significantly deviated from Hardy-Weinberg equilibrium, but the average of the F s (?) does not deviate from Hardy-Weinberg equilibrium (F s (?) = 0.037, P> 0.05). Nei's genetic similarity coefficient is high (0.990 ~ 0.996), indicating that, despite the three populations exist between geographic isolation, but the genetic differentiation between them was relatively small. In order to Roger's genetic distance-based clustering analysis, the distance closer yangmingbu County, Shanxi Taiyuan Jinyuan populations and populations together first and then with Shaanxi Chang'an County Yangqiao populations together, indicate three Oxya locust population genetic distance and geographic distance between the location and obvious correlation between the ending. However, the amount needed to detect human populations in order to support the current results. The correlation between genotype a toxicity analysis results show that the selective allozyme loci Oxya genotype and lethal effects of malathion was significantly correlated. Two Oxya exists between population genetic differentiation, although this did not reach statistical differentiation significant difference (only 1 = 0.037 ± 0.020, corpse gt; 0.05), but this difference has led to different populations Acute toxicity of malathion reactions behave differently. Shaanxi Chang'an County Yangqiao corpse rural populations and Shelob n a bb cc genotype mortality knife a significant difference, rejection,: a bb genotype Oxya malathion toxicity of the individual there is a large tolerance sex (49% mortality), and assistance. A cc genotype Oxya individual Quedui malathion toxicity manifested very sensitive (68% mortality). Further, in this population, allele assistance. A b and g sub a corpse, an a, Shelob, and the corpse of a foot an d there were significant differences between, D.. Has a high frequency of b allele PLvln an individual right Mara Oxya sulfur Phosphorus toxicity there is a big tolerance; Hill generations County pieces! Fort populations coffin out of a move and i bb genotype Pgi a significant difference between the mortality rate, Pgi an individual ab genotype Oxya malathion there is a big toxicity tolerance (52%), while Pgi a bb genotype Oxya malathion toxicity Quedui individual performance is very sensitive (64%); Taiyuan Jinyuan Town Oxya individual Pgl ' -c. And dead legs a a. Are associated with lethal malathion was significantly associated with rejection and co n i a c'c an ac genotype BU to all survived, while the other Pgi genotype had the lowest mortality was 48%, spoon. Other genotype mortality is at least 46%, while in the population, who mediated an d the highest mortality rate, with people called an a, e a b a c and human mediated mortality, there were significant between the difference. Roger's genetic distance showed that malathion acute toxicity of each Oxya population genetic structure are present differentiation.