The Study of the Relationship Expression of NF-κ Bp65, CDK4, MMP-9, TIMP-2 with Malignant Degree and Relapse of Astrocytoma

Objective To explore the expression of NF－κBp65，CDK4，MMP-9 and TIMP-2 in astrocytoma,and investigate the relevance of NF－κBp65，CDK4，MMP-9 , TIMP-2 and several clinical factors in tumor malignant degree and relapse.Methods Fifty-four final diagnosis human astrocytoma (13 case of astrocytoma grade I,16 case of astrocytoma grade Ⅱ,14 case of astrocytoma grade Ⅲ,11 case of astrocytoma grade Ⅳ).Normal brain tissues were from 10 case of internal decompression of brain trauma.The SP(Methods Streplavidin-Petvxidase) immunohistochemical staining method was used to examine the expression of NF－κBp65，CDK4，MMP-9 and TIMP-2 in 54 human astrocytoma and 10 Normal brain tissues,the factors that can affect relapse we reevaluated.Results The expression of NF－κBp65，CDK4 and MMP-9 was <WP=10>significantly different in distinct grade astrocytoma(P＜0.05)， But expression of TIMP-2 was no different in distinct grade astrocytoma(P＞0.05).In the astrocytoma ,expression of NF－κBp65 was related to expression of CDK4 (r=0.5694,P＜0.05), also related to expression of MMP-9 (r=0.7261, P＜0.05),and expression of CDK4 was also related to expression of MMP-9(r=0.6397, P＜0.05).There was no relational between expression of MMP-9 and TIMP-2 (r=0.0567,P=0.684＞0.05).The patients’age,tumor resections coverage,the malignant degree,radiotherapy,the expression of NF－κBp65，CDK4，MMP-9 and TIMP-2 were correlated with relapse rate of one year by simplex factor Logistic analysis.The results of multivariate logistic analysis showed that the CDK4,MMP-9 and TIMP-2 expression were significantly correlated with relapse.In astrocytoma,the expression of CDK4 and MMP-9 was risk factor of tumor relapse,but the expression of TIMP-2 show as protective factor.Conclusions The expression of NF－κBp65，CDK4 and MMP-9 were significantly correlated with astrocytoma malignant,but not TIMP-2.The NF－κBp65，CDK4 and MMP-9’s expression were recrudescent markers of astrocytoma.As the still deeper research of NF－κBp65，CDK4，MMP-9 and TIMP-2 was <WP=11>accomplished,a new way which astrocytoma is treated by gene therapy will be found.