The Effect of Adiponectin and Leptin and Body Composition on Bone Mass Loss Induced by Exercise
|School||Capital Institute of Physical Education|
|Course||Human Movement Science|
|Keywords||Movement Bone metabolism Adiponectin Leptin Bone mineral density Body Composition Estrogen E2|
Objective: Incremental Exercise 3-month-old female SD rats, to establish the amount of movement of bone to reduce the rat model; analysis of bone mineral density, serum estrogen (E2), body composition and other indicators changes and immunohistochemistry (immunohistochemistry, IHC-P) analysis the rat right distal femoral epiphysis line area adiponectin (Adiponectin, AdipoQ), leptin (leptin) expression into bone cells (osteoblast, OB), explore the finding that a variant of the ADIPOQ, leptin and body composition indicators in exercise bone and reduce the role and mechanism. Methods: 190 3-month-old female SD rats (Sprague Dawley rat) were randomly divided into control group 90 and exercise group (n = 100). Exercise group Treadmill Desk training, the control group without training. All rats a week for four consecutive days of vaginal cytology, observed estrous cycle change. In 4 weeks, 9 weeks, 13 weeks, 15 weeks, 17 weeks were drawn after the rats were anesthetized and whole body bone mineral density scan and serum E2 detection, take the right distal femur, decalcification, paraffin-embedded sections, immunohistochemistry assay AdipoQ, Leptin expression in osteoblasts. Results: 1,4-week exercise group rats BMD than the control group significantly increased (P lt; 0.05), 13-week exercise group rats BMD is significantly lower than the control group (P lt; 0.05) 15 weeks and 117 weeks of exercise group BMD than the control group was significantly decreased (P lt; 0.01) 2, 13 weeks, 15 weeks, 17 weeks sports serum E: concentration lower than the control group, there was significant difference (P lt; 0.01). 3,9 weeks, 13 weeks, 15 weeks, 17 weeks exercise group percentage of body fat than the control group, there was significant difference (P lt; 0.01); 4 weeks, 9 weeks, 13 weeks, 15 weeks, 17 weekly exercise group the rat peritoneal fat weight than the control group, there was significant difference (P lt; 0.01). Right distal femur of the exercise group as the bone cells AdipoQ in four weeks, 9 weeks, 13 weeks, 15 weeks, 17 weeks staining gradually become darker with increasing exercise load, in the process of long-term load movement of the finding that a variant of the ADIPOQ expression increased; Leptin four weeks, 9 weeks, 13 weeks, 15 weeks, 17 weeks dyeing faded reduce leptin expression with the increase of exercise load in the process of long-term load movement. Staining of the control group did not change significantly. Conclusion: After 17 consecutive weeks of incremental training, excessive exercise bone mass to reduce animal model successfully established. 2, long-term load exercise HPO axis suppression, reduced E2 level is an important factor in this process lead to reduced bone mass. Leptin involved in the regulation of bone metabolism during long-term high-load exercise reduced bone mass.