The Study about the Recovery of Nerves Function in Rats of Spinal Cord Injury by Step Training
|School||Capital Institute of Physical Education|
|Course||Human Movement Science|
|Keywords||Spinal cord injury Walking training BBB motor scores Reuter sensory function score BDNF NT-3|
Objective: To observe walking training on spinal cord injury in rats with nerve function, and brain-derived nerve growth factor in the injured spinal cord gray matter anterior horn (brain derived neurotrophic factor, BDNF) and neurotrophin -3 (neurotrophin-3, NT-3 ) expression were determined. Elaborated the role of BDNF and NT-3 promote walking training in rats with spinal cord injury recovery of neurological function. : 1 of 26 3-month-old female SD rats were randomly divided into damage control group (A group), walking training group (B), n = 13. 2 NYU spinal cord in the fight against falling height of 25mm against T9 spinal cord causing moderate injury model in rats, a week after the injury walking training group received walking training intervention, injury control group without intervention. Modeling weekly motor function BBB rating and Reuter sensory function score of neurological function were evaluated. 4 immunochemistry and Western blotting was used to detect walking training of BDNF and NT-3 expression in rats with spinal cord injury spinal cord, analyze its relationship with the neurological function recovery. Results: BBB movement function score results: 3 weeks after (the walk after 2 weeks), group A group B with the same point in time comparative rating has improved significantly. Scores in three weeks, a significant difference (P lt; 0.05), group B (5.7 ± 1.03), group A (4.4 ± 0.77) min. 4 thoughtful week 8 scores has a very significant difference (P lt; 0.01), week 9 rated value is significant difference (P lt; 0.05), group B (11.0 ± 1.06) min A group (9.7 ± 0.87) points. Self-control score of group B comparison between the first 2 weeks to 8 weeks difference 8 weeks and 9 weeks there was no difference in the scores of the week 8 (11.8 ± 0.83). A control group rated between the 6th week of the first 3 weeks compared differences, no difference 6 weeks after the first 6 weeks score (8.7 ± 0.49) stars. The Reuter sensory function score results: A group compared with the same point in time in group B score after 4 weeks (3 weeks after walking training) began to have differences, differences continued until 8 weeks after surgery, the first of which 4,5 6,7 weeks score with a very significant difference (P lt; 0.01). Rating in the first nine weeks is not a difference in group B (1.9 ± 0.31), group A (2.8 ± 0.79). Group B control score from the first 3 weeks to seven weeks between more differences, there was no difference in the seven weeks after Week 7 scores (2.5 ± 0.53) min. A control group score from between the 6th week of the first 4 weeks compared difference, having no difference 6 weeks after the first 6 weeks score (4.3 ± 0.61) stars. 3 BDNF Western blotting assay results A group and B group compared with the very significant difference (P lt; 0.01). NT-3 Western blotting assay results in group A compared with group B also has a very significant difference (P lt; 0.01). 4 exercise group BBB score Reuter score correlation coefficient r = -0.742, to test the correlation coefficient r, P lt; 0.01, a negative correlation between the BBB score and Reuter rated. Prompt recovery of motor function and sensory function recovery relevant. 5BDNF BBB score correlation coefficient r = 0.868, to test the correlation coefficient r, P lt; 0.01, a positive correlation between BDNF and BBB rated. BDNF and Reuter score correlation coefficient r = -0.644, to test the correlation coefficient r, P lt; 0.05, a negative correlation between BDNF and Reuter score. Tip walking training by promoting the increase in the number of BDNF to improve neurological function. 6 NT-3 and BBB score between the correlation coefficient r = 0.634, to test the correlation coefficient r, P lt; 0.05, a positive correlation between NT-3 and BBB rated. NT-3 and Reuter score correlation coefficient r = -0.656, to test the correlation coefficient r, P lt; 0.05, a negative correlation between NT-3 and Reuter rated. Tip walking training can be improved through the promotion of NT-3 increase in the number of rats with nerve function. Conclusion: walking training can promote functional recovery after spinal cord injury in rat neural mechanism may promote the surrounding tissue to BDNF and NT-3 expression in the spinal cord injury site and walking training.