Effect of Radiofrequency Ablation on Tumor Cell Apoptosis in Rabbit Tumor Model
|Keywords||Radiofrequency ablation Hepatic carcinoma Apoptosis|
Objective: Radiofrequency ablation as a ablation has been widely used in the treatment of liver cancer , and achieved good clinical results , but the treatment of radiofrequency ablation of liver tumors mechanism is unclear . In this study, by observing the histopathological changes of the surrounding tumor tissue after radiofrequency treatment , to understand the RF treatment with apoptosis . Methods : 32 rabbits , VX 2 tumor cells implanted liver left central leaf made ??of liver cancer model . The rabbits were divided into two groups randomly , the RF group ( 28 ) and control group ( 4 ) , the RF group given 24 days after feeding RF treatment , and after 0,2,6,12,24,48,168 h , from sacrificed control group fed for 24 days after the drawn killed . By the naked eye , microscopy and transmission electron microscopy after each of the tumor tissue and RF phase surrounding the tumor histopathology changes, using the TUNEL assay apoptosis index changes , and diphenylamine assay DNA degradation index changes . Results: RF tumor coagulation necrosis after radiofrequency ablation , coagulation necrosis the marginal zone tumor tissue can be seen significant changes in apoptosis and necrosis . TEM can be observed in the edge area there is a large number of apoptotic cells and necrotic cells . RF edge of the area after tumor cell apoptosis index than RF tumor tissue was significantly increased (P <0.01), reached the peak at 12h and 168h after the reduction , but still higher than the RF front level ( P <0.01 ) . Marginal zone tumor DNA degradation index by RF 2h elevated tumor tissue than RF ( P <0.05 ) , reached the peak at 12h , then decreased gradually 168h was no significant difference ( P > 0.05 ) and RF front Conclusion: Radiofrequency treatment induced hyperthermia coagulation necrosis area surrounding the tumor cell apoptosis is an important mechanism of RF treatment of malignant tumors .