Dissertation > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors

Effect of Radiofrequency Ablation on Tumor Cell Apoptosis in Rabbit Tumor Model

Author WangDiLe
Tutor ShenShiQiang
School Wuhan University
Course General Surgery
Keywords Radiofrequency ablation Hepatic carcinoma Apoptosis
CLC R735.7
Type Master's thesis
Year 2004
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Objective: Radiofrequency ablation as a ablation has been widely used in the treatment of liver cancer , and achieved good clinical results , but the treatment of radiofrequency ablation of liver tumors mechanism is unclear . In this study, by observing the histopathological changes of the surrounding tumor tissue after radiofrequency treatment , to understand the RF treatment with apoptosis . Methods : 32 rabbits , VX 2 tumor cells implanted liver left central leaf made ??of liver cancer model . The rabbits were divided into two groups randomly , the RF group ( 28 ) and control group ( 4 ) , the RF group given 24 days after feeding RF treatment , and after 0,2,6,12,24,48,168 h , from sacrificed control group fed for 24 days after the drawn killed . By the naked eye , microscopy and transmission electron microscopy after each of the tumor tissue and RF phase surrounding the tumor histopathology changes, using the TUNEL assay apoptosis index changes , and diphenylamine assay DNA degradation index changes . Results: RF tumor coagulation necrosis after radiofrequency ablation , coagulation necrosis the marginal zone tumor tissue can be seen significant changes in apoptosis and necrosis . TEM can be observed in the edge area there is a large number of apoptotic cells and necrotic cells . RF edge of the area after tumor cell apoptosis index than RF tumor tissue was significantly increased (P <0.01), reached the peak at 12h and 168h after the reduction , but still higher than the RF front level ( P <0.01 ) . Marginal zone tumor DNA degradation index by RF 2h elevated tumor tissue than RF ( P <0.05 ) , reached the peak at 12h , then decreased gradually 168h was no significant difference ( P > 0.05 ) and RF front Conclusion: Radiofrequency treatment induced hyperthermia coagulation necrosis area surrounding the tumor cell apoptosis is an important mechanism of RF treatment of malignant tumors .

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