The Analysis of Active Chemicals in Yinchenhao Decoction with Hepatocyte Membrane Immobilized Chromatography
|School||Nanjing University of Traditional Chinese Medicine|
|Keywords||YCHT Liver cell membrane solid-phase chromatography Active ingredients|
From YCHT \Modern research has shown that it has a gallbladder, liver, retaining pancreas, antipyretic and anti-inflammatory analgesic, antibacterial and antiviral pharmacological effects. Geniposide, 6,7 - dimethoxy coumarin has been shown as the main active ingredient, but the party still active ingredient research gaps. Established liver cell membrane immobilized of that party biochromatography hepatoprotective effect effect ingredients to carry out a preliminary study. First part of this article mentioning alcohol precipitation liquid water YCHT and HL-7702 normal human liver cell lines for the study, identified YCHT HL-7702 normal human liver cell line security scope, test results show that the : Yinchenhao soup extraction and ethanol precipitation solution concentration range can be controlled within 5 ~ 0.001mg crude drug / ml range, no cytotoxicity liver cell line HL-7702. Extraction and ethanol precipitation liquid Yinchenhao soup final concentration of greater than 10mg Pharmacognostic / ml toxic effects significantly (P <0.001), liver cell line HL-7702, resulting in cell death after 24 hours; with blank control group compared, the final concentration of 5 mg crude drug / ml Yinchenhao soup alcohol Shen liquid no inhibitory effect on the growth of the HL-7702 human liver cell line; final concentration of 0.1 ~ 1mg crude drug / ml Yinchenhao soups alcohol Shen solution can significantly promote the HL-7702 human liver cell line growth (P <0.05). Therefore, the concentration is determined to be 0.1 ~ 1mg crude drug / ml. Extraction and ethanol precipitation liquid Yinchenhao soup was observed in the concentration range of the protective effect of normal human liver cell line HL-7702 D-GalN injury in the second part of this article. The results showed that: capillaris mentioning Artemisia soup, alcohol precipitation liquid with lower D-GalN induced liver cell supernatant ALT, AST increase. The can protect YCHT D-GalN induced liver cell damage. The third part of this paper the binary nonlinear gradient elution RP-HPLC spectra YCHT. The study showed that: the optimal detection wavelength of 254nm, 280nm, 310nm, the best record time of 55 minutes, the column temperature was 25 ° C. Get Yinchenhao soup extraction and ethanol precipitation liquid solid phase high performance liquid spectra as liver cell membrane chromatography. In this paper, liver cell membrane immobilized analysis Yinchenhao soup extraction and ethanol precipitation liquid chromatography with HL-7702 normal human liver cell membrane specific binding of the chemical composition, preliminary studies have shown that the four characteristic components of the HL-7702 liver cell membrane specific binding , while the four components derived from rhubarb.