Response of Glycine Soja, Glycine Max and Its Hybrid Descendants to the Chloride (Salt) Stress
|School||Nanjing Agricultural College|
|Keywords||Wild soybean Cultivated soybean Hybrids Isotonic Na ~ - salt Cl ~ - salt|
Wild soybean populations BB52 (salt tolerance) and N23232 (salt weaker) cultivated soybean Lee68 (salt tolerance) and Jackson, the N23674 (salt tolerance weak) and BB52 with Jackson hybridization F4 generation of BJ185 lines for materials, isotonic (-0.53MPa) PEG-6000, NaCl, the of Na sup> and Cl - sup> - salt (all 120mmol.L -1 sup>) of its seeds and seedlings, experimental results are as follows: NaCl Stress under wild and cultivated seed germination rate decreased significantly, PEG-6000 treatment, both wild and cultivated soybean seed germination rate of the control is no obvious difference , but visible the Wild Soybean stronger ability to adapt to osmotic stress. Terms of Na sup> - sup> - salt treatment - salt and Cl Na sup> - salt treatment germination rate is more obvious. For the test of wild soybean and cultivated soybean seedlings under NaCl stress, leaf relative electrolyte leakage, MDA content significantly increased chlorophyll fluorescence parameters Fv / Fm and ETR values ??decreased significantly, PEG-6000 treatment under the above did not change significantly small. The trend NaCl treatment of the na - salt and Cl sup> - sup> - salt treatment under the above indicators are basically the same, and wild soybean populations Na sup> salt treatment under the above physiological indicators of change the obvious salt treatment, while the change in the cultivation of soybean in the isotonicity Cl - sup> - salt treatment under the above indicators compared with isotonic of Na Cl - sup> - < sup> sup> salt obvious. NaCl treatment, the cultivated soybean Pn (net photosynthetic rate) significantly decreased; PEG-6000 treatment under Pn also declined, but not as good as NaCl treatment significantly. Na sup> - salt and Cl - sup> - the salt treatment Pn value in NaCl and PEG-6000, Na sup> - salt treatment Pn value than Cl - sup> - high salt treatment. NaCl, Na sup> of salt and Cl - sup> - salt treatment, Gs (stomatal conductance) value decline accompanied by Ci (intercellular CO 2 concentration) elevated, PEG-6000 treatment under Gs and Ci decreased. The isotonic PEG-6000 treatment cultivation the soybean leaf Rubisco activity of the control was no significant difference. NaCl, Na sup> of salt and Cl - sup> - the salt processing under processing, Rubisco activity was significantly decreased Cl - sup> - salt treatment Rubisco enzyme activity decreased Na sup> - salt is NaCl treatment showed Cl - sup> and Na sup> cumulative effect. The Cl - sup> - salt processing chloroplasts Cl - sup> and Na sup> of the total content of greater than Na sup> salt treatment. NaCl treatment of the chloroplasts Cl - sup> and Na sup> total content. The results showed that the wild soybean is more sensitive Na sup>, and the cultivation of soybeans are more sensitive Cl - sup>. 50,100,200 mmol / L NaCl treatment the cultivated soybean Jackson (weak salt tolerance) and wild soybean the BB52 and their hybridization F4 generation BJ185 seedling stage (already Salt screening), found that as the salt concentration increased, The hybrid offspring BJ185 leaves Relative electrolyte leakage, MDA content and chlorophyll content changes in between the two parents. In the the above different isotonic processing, BJ185 seedlings and wild soybean BB52, electrolyte leakage and MDA content in Na sup> - salt treatment increased the most obvious. BJ185 and wild soybean BB52 in of Na sup> - salt treatment of free putrescine (Put) rising obvious than the isotonicity Cl - sup> - salt while Cl - sup> - salt treatment free spermidine (Spd) rise greater than isotonic Na sup> - the salt handling Cl , - sup> - salt treatment (Spd Spm) / Put value greater than Na sup> - salt treatment. Cl - sup> - bound state putrescine under salt treatment increased greater than Na sup> - salt processing, bound states spermine (Spm) and spermidine content is no obvious difference. The above results show that the hybridization between the salt-tolerant populations of wild soybean and cultivated soybean varieties, hybrids BJ185 salt tolerance has been improved. The NaCl stress ion effect, BJ185 and BB52 Cl - sup> than Na sup> stronger performance patience. With Cl - sup> - salt treatment they can maintain a higher (Spd Spm,) / Put value and accumulated more bound polyamine-related.