Expression and Significance of SUMO-1 in Adjuvant-induced Arthritis Mouse and Influence of ～(99)Tc-MDP
|School||Central South University|
|Course||Internal Medicine Rheumatology Diseases|
|Keywords||adjuvant-induced arthritis(AA) small ubiquitin-like modifier-1 (SUMO-1) 99Tc-MDP Immunohistochemistry Assay|
Objective To examine the expression of small ubiquitin-like modifier-1（SUMO-1）in adjuvant-induced arthritis （AA） mouse and the influence of 99Tc-MDP.Methods The AA was established by subcutaneous injection with complete Freud’s adjuvant to rats, except the normal group A（n=8）. The rats successfully induced AA（n=18） were divided into two groups randomly:①Model group B（n=8, treated with normal saline）;②99Tc-MDP group C(n=10, treated with 99Tc -MDP); The symptoms of arthritis were evaluated by arthritis index; the histopathological （hematoxylin and eosin stain） changes of joint were scored in infiltration of inflammatory cells and hyperplasia of synoviocytes. The expressions of SUMO-1 in synovium and joint were detected by Immunohistochemistry technique.Results 1. Arthritis index（AI）: The arthritis indexes of model group and 99Tc-MDP group were increasing with the time elongation. There were no significant diferences in the first 36 days, after which the arthritis indexes of model group became higher than 99Tc-MDP group （P<0.05）. 2. The histopathological score: The histopathological scores of model group were significantly higher than 99Tc-MDP group（P<0.05）. 3. Immunohistochemistry Assay: The levels of SUMO-1 of model group and 99Tc-MDP group were notably higher than those of normal group （P<0.05）. The levels of SUMO- 1 of model group were notably higher than those of 99Tc -MDP group（P<0.05）. 4 The correlation between SUMO-1 and the level of hyperplasia of synoviocytes and AI were very good.Conclusion The expression of SUMO-1 in AA mouse with severe arthritis is significantly increased, and it is correlated with disease active index and level of hyperplasia of synoviocytes. SUMO-1 may be one of important factor in the pathogenesis of rheumatoid arthritis （RA）. 99Tc -MDP can comparatively decrese the levels of SUMO-1, and it may induce apoptosis of synoviocytes.