Dissertation > Medicine, health > Internal Medicine > Systemic disease > Autoimmune diseases > Autoimmune diseases, connective tissue disease

Expression and Significance of SUMO-1 in Adjuvant-induced Arthritis Mouse and Influence of ~(99)Tc-MDP

Author GuoMin
Tutor ZuoXiaoXia
School Central South University
Course Internal Medicine Rheumatology Diseases
Keywords adjuvant-induced arthritis(AA) small ubiquitin-like modifier-1 (SUMO-1) 99Tc-MDP Immunohistochemistry Assay
CLC R593.2
Type Master's thesis
Year 2007
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Objective To examine the expression of small ubiquitin-like modifier-1(SUMO-1)in adjuvant-induced arthritis (AA) mouse and the influence of 99Tc-MDP.Methods The AA was established by subcutaneous injection with complete Freud’s adjuvant to rats, except the normal group A(n=8). The rats successfully induced AA(n=18) were divided into two groups randomly:①Model group B(n=8, treated with normal saline);②99Tc-MDP group C(n=10, treated with 99Tc -MDP); The symptoms of arthritis were evaluated by arthritis index; the histopathological (hematoxylin and eosin stain) changes of joint were scored in infiltration of inflammatory cells and hyperplasia of synoviocytes. The expressions of SUMO-1 in synovium and joint were detected by Immunohistochemistry technique.Results 1. Arthritis index(AI): The arthritis indexes of model group and 99Tc-MDP group were increasing with the time elongation. There were no significant diferences in the first 36 days, after which the arthritis indexes of model group became higher than 99Tc-MDP group (P<0.05). 2. The histopathological score: The histopathological scores of model group were significantly higher than 99Tc-MDP group(P<0.05). 3. Immunohistochemistry Assay: The levels of SUMO-1 of model group and 99Tc-MDP group were notably higher than those of normal group (P<0.05). The levels of SUMO- 1 of model group were notably higher than those of 99Tc -MDP group(P<0.05). 4 The correlation between SUMO-1 and the level of hyperplasia of synoviocytes and AI were very good.Conclusion The expression of SUMO-1 in AA mouse with severe arthritis is significantly increased, and it is correlated with disease active index and level of hyperplasia of synoviocytes. SUMO-1 may be one of important factor in the pathogenesis of rheumatoid arthritis (RA). 99Tc -MDP can comparatively decrese the levels of SUMO-1, and it may induce apoptosis of synoviocytes.

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