Dissertation > Biological Sciences > Microbiology > Microbial Genetics

Bioinformatic Study on Genome of Radiation Resistant Bacterium Deinococcus Radiodurans

Author SongQin
Tutor ZhangHongYu
School Shandong University of Technology
Course Biochemistry and Molecular Biology
Keywords Deinococcus radiodurans Genomic analysis Predicted highly expressed gene
CLC Q933
Type Master's thesis
Year 2007
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Deinococcus radiodurans is a non-spore Gram bacterium which can yiled red pigment. It can resist ionize radiation, UV radiation gamma, radiation and other DNA damage substance.We analyze the genomes of the most radiation resistant bacterium Deinococcus radiodurans and other sixteen non-radiation resistant microbes which GC content are from 65% to 69% and growth temperature from 20°C to 40°C. We try to esplore the Deinococcus radiodurans’radiation resistant mechanism from the content of animo acid and protein structure. The radiation resistant bacterium Deinococcus radiodurans’3.28 megabase high G+C genome contains two chromosomes, a megaplasmid and a small plasmid.By comparison analysis of amino acid usage bias of seventeen microbes , we conclude that in Deinococcus radiodurans’genome, Proline (P) is higher than others, and Asparagine (D), Isoleucine (I) is lower. The index RAI can reflect the animo acid usage bias of the radiation resistant bacterium Deinococcus radiodurans and other sixteen non-radiation resistant microbes. And by analysis of secondary structure contents (helix; pleated sheet; random coil and turn), we conclude that in the most radiation resistant bacterium Deinococcus radiodurans genome. The content of C is higher than other sixteen non-radiation resistant microbes.We analized the genomes of five radiation resistant microbes including Deinococcus radiodurans using Karlin’s index of condon usage. It is revealed that ribosomal proteins S3 and S12 are predicted highly expressed (PHX) in all of the five genomes. In addition, some genes coding transcription/translation and chaperones: infB RpoC fusA dnaK groEL and GroES are predicted to be highly or marginally highly expressed. Some proteins associated with cell surface structure and a number of ABC transporters are PHX in some species. However, most proteins associated with direct repair, base excision repair, apurinic/apyrimidinic (AP) endonuclease, mismatch excision repair, recombinational repair (escept recombinase A (RecA) gene) and other genes of repair enzyme show low expression levels.

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