Experimental and Clinical Study of Anticaious Activity of Propolis Toothpaste
|School||Chongqing Medical University|
|Keywords||Propolis Toothpaste Plaque pH value Anticaries|
Background and Purpose: Caries (Dental Caries) is under the influence of multi-factor based bacteria, dental hard tissue chronic destruction of a disease is the most common form of oral diseases. The World Health Organization has been linked to cancer and cardiovascular disease is tied for the three diseases that endanger human health. The plaque is indispensable in the process of caries microecological environment. Plaque bacterial metabolism of carbohydrates to produce acidic metabolites can cause enamel demineralization, dissolved, and thus the formation of dental caries. Therefore, as long as they can effectively control the plaque, it can effectively control the occurrence and development of dental caries. Drug toothpaste as one of the methods to control the plaque has been widely used in oral self-care measures, to add appropriate anti-caries drugs can be used as an ideal anti-caries ways toothpaste. In recent years, the natural medicine as an alternative healthcare has become an important strategy in the development of new drugs. Propolis as a natural product of a wide range of pharmacological effects, can inhibit the growth and adhesion of cariogenic bacteria, and can effectively control the plaque formation and reduce the incidence of caries. According to the literature propolis toxic side effects, safe to use, and do not develop resistance. Research on propolis, propolis toothpaste reported rare. Propolis toothpaste, control the plaque through daily brushing, along with the dual role of the chemical and mechanical control, has broad prospects for development. This study in vitro antibacterial experiments with domestic propolis ethanol extract (EEP), on the basis of the development of a propolis toothpaste, the antibacterial experiments vitro and in vivo clinical trials, the observed antimicrobial effect on plaque formation and plaque pH changes, and to explore the mechanism of propolis against dental caries, provided the experimental basis for the clinical development of propolis. Materials and methods, propolis alcohol solution with 95% ethanol, 1:2-fold dilution method, dubbed the concentration of the liquid, under anaerobic conditions, cultured Streptococcus mutans with light spit agar medium (MSA), and The Streptococcus Sobrinus. Kirby-Bauer disk diffusion method, measuring the size of the inhibition zone to determine propolis two Streptococcus minimum inhibitory concentration (MIC). 2, using the disk diffusion method, as a negative control blank toothpaste, 0.2% chlorhexidine solution as a positive control, observations and 5%, 2.5%, 1% and 0.5% propolis toothpaste on Streptococcus mutans and distant chain cocci inhibition. 3, using a completely randomized control methods, select 40 healthy volunteers were randomly divided into two groups before the experiment detected baseline plaque index (PI0) blank the toothpaste (excluding propolis) and 1% propolis toothpaste brushing every 2 times a day, one week and four weeks after the review, were detected by plaque index PI1 and PI4, evaluation propolis toothpaste plaque formation clinical effect. 4, 10 healthy young volunteers, select the right mandibular first two premolar interproximal plaque as a test site, require this part of the healthy tooth surface. Using their own before and after the control study, self-made miniature pH electrode in situ detection rinse with 10% sucrose solution before and after 1 hour (0,2,5,10,15,20,30,40,50,60 min 10 time points) plaque pH of dynamic change, and compare observed after 4 weeks containing propolis toothpaste without propolis toothpaste, plaque pH change of one hour before and after the rinse sugar. 1, alcoholic solution of propolis on Streptococcus mutans and distant streptococci are good inhibition, minimum inhibitory concentration of 0.3125% (3.125mg/ml) and 0.625% (6.25 mg) and inhibition with propolis concentration increased enhance. 2, various concentrations of propolis toothpaste can effectively inhibit the growth of Streptococcus mutans and Streptococcus sobrinus, its inhibition has a significant concentration-dependent manner; 5% propolis toothpaste on the inhibition of Streptococcus mutans and chlorhexidine suppression bacteria of the same (P gt; 0.05), and of the Streptococcus sobrinus inhibition with chlorhexidine close. Use toothpaste after four weeks, the two groups of PI values ??compared to the first week has decreased, with significant difference (P lt; 0.05) between the two groups; compared two groups before and after their first four weeks of the experimental group PI variation (? PI4) 1 week PI variation (? PI1), with a significant difference (P lt; 0.05). 4, has two toothpaste after four weeks, the plaque pH variation rinse sugar baseline Stephan curve similar rising trend after it was reduced. 4 weeks with propolis toothpaste brushing rinse sugar plaque pH has not consistently below the critical pH of 5.5 or less, and between 5 and 10min time point pH and the parameters (pHmin and? PH) and baseline Stephan curve statistically difference (P lt; 0.05). Conclusion 1, propolis on the growth of Streptococcus mutans and Streptococcus sobrinus has good inhibition shows propolis antibacterial anticaries performance. Propolis toothpaste can effectively inhibit the growth of Streptococcus mutans and Streptococcus sobrinus and has a significant concentration-dependent manner; 5% propolis toothpaste antibacterial chlorhexidine close of. Propolis toothpaste control dental plaque better than without propolis blank toothpaste; prompts using propolis toothpaste can effectively control supragingival plaque formation, caries prevention. 4, after the use of propolis toothpaste four weeks, can reduce the amplitude of plaque pH changes in the intake of sugar, indicating that propolis through toothpaste form applied to the oral partial, affecting the metabolism of plaque acid production, achieve the role of prevention of dental caries.