Dissertation > Medicine, health > Clinical > Therapy > Oxygen therapy,hyperbaric oxygen therapy

The Effectiveness and Mechanism of Cerebral Ischemic Tolerance Induced by Hyperbaric Oxygen

Author GuGuoJun
Tutor SunXueJun;LiRunPing;XuWeiGang;TaoHengYi
School Second Military Medical University
Course Aviation, aerospace and maritime medicine
Keywords Hyperbaric oxygen Cerebral ischemic tolerance Hypoxia-inducible factor-1 Erythropoietin
CLC R459.6
Type Master's thesis
Year 2007
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[Objective] To investigate whether HBO in rats induced ischemic tolerance and its possible mechanism . [Methods] SD rats with HBO (100% O2, 2ATA, 1h / times , every other day × 5 times ) or atmospheric air after pretreatment . The use of endothelin -1 (endothelin-1, ET-1) to establish cerebral ischemia model by neurological score , brain water content determination , 2,3,5 , . Triphenyl tetrazolium ( 2,3 , 5-triphenyltetrazolium chloride, TTC) staining , HE staining methods, evaluation HBO -induced ischemic tolerance effect ; addition , the application of RT-PCR, Western blot and ELISA-based kit was used to detect HBO preconditioning on the cortex and hippocampus HIF-la and its target gene expression. [Results] HBO preconditioning can significantly promote cerebral ischemia and nerve function recovery , cerebral edema , significantly reduced infarct volume and significantly reduce brain tissue damage ; HBO pretreated rat cerebral cortex and hippocampus tissues the HIF-1amRNA, protein content and DNA binding activity was significantly enhanced , HIF-1 target gene EPO mRNA and protein content was significantly increased, while VEGF, Glut-1 mRNA showed no significant change. [Conclusion] repeatedly HBO preconditioning can induce ischemic tolerance , one of the mechanisms may be: Repeated HBO preconditioning cause the body HIF-1 and downstream neuroprotective genes ( such as EPO) expression of neuroprotective effect.

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