Dissertation > Medicine, health > Ophthalmology > Ocular adnexal organ disease

Research on the Effect and Mechanism of Chitosan to Dry Eyes

Author LiQin
Tutor YangHong
School Huazhong University of Science and Technology
Course Ophthalmology
Keywords Chitosan The rabbits dry eye model Mucin Muc-5ac Immunohistochemistry Western Blot.
CLC R777
Type Master's thesis
Year 2006
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Objective To explore the chitosan mechanism of action of the treatment of dry eye . 32 New Zealand white rabbits (weighing 2 ± 0.5 Kg) were randomly divided into normal control group , dry eye group , chitin Sugar group and tears ran group . Production of animal models of dry eye , the chitosan group and the tears ran group topical 2% PBS solution with chitosan solution and tears ran topical eye drops , dry eye group and normal control group . 1, 2, 3, and 4 weeks after the medication , and observe the change of appearance of each group hotels Tujiao conjunctival , simultaneous detection of the Schirmer test value , but values ??. Each group were four eyes, the animals were sacrificed at each time point , take the conjunctival tissue slices Immunohistochemistry was used to detect the conjunctiva the Muc-5ac protein expression changes . And the first 4 weeks after the administration , each group were conjunctival tissue homogenates extracted protein solution , semi-quantitative detection with the Western Blot method wherein protein Muc-5ac . Chitosan group and the tears ran group after three weeks of topical Schirmer test values ??and BUT values ??than those of dry eye group increased significantly (P lt; 0.01). Chitosan group and tears contingent group after treatment compared with dry eye syndrome group conjunctival epithelial mucin Muc - 5ac grayscale value , protein bar band optical density (OD) increased ( P lt; 0.05 ) , and similar to the normal control group ( P gt; 0.05). Chitosan group with tears contingent group Schirmer test , BUT value , conjunctival epithelial mucin Muc - 5ac gray value , protein bands of the optical density (OD) of all indices showed no significant difference (P gt; 0.05). Conclusion Chitosan can promote conjunctival epithelial ( goblet cells ) to produce mucin MUC-5AC treatment of dry eye .

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