Effect of Glucose, Sucrose and NaCl to Embryo Development of Zebrafish
|School||Shandong Normal University|
|Keywords||Glucose Sucrose Sodium chloride Zebrafish Embryonic development|
Pure fresh water is freshwater fish ideal living conditions? Increase water to the appropriate concentration of inorganic salts, nutrients, neutral, non-toxic organic ingredients, and the osmotic pressure caused by these different substances appropriate conditions on freshwater fish life and embryonic development, what kind of impact it? Currently, little is known about these problems. Fish at the stage of embryonic development is most sensitive to changes in environmental conditions, and to observe the impact of different environmental conditions on fish embryo development is also a shortcut to study the effects of environmental conditions on fish. This experiment, early zebrafish embryos (about 2 hours), and after fertilization, were cultured in a solution of different concentrations of glucose, sucrose, sodium chloride, etc., compared with the parallel freshwater in the control group were observed environmental conditions on zebrafish embryonic development. No obvious side effects to the to explore appropriate concentration of salt and sucrose and glucose (can also be considered to be a class of nutrients) its osmotic pressure and other conditions of zebrafish embryos to produce positive (or negative) impact Improved the zebrafish embryos conditions, to improve the quality of embryo development, the high concentration of the solution, even if it is non-toxic side effects of sucrose or glucose, because they cause the osmotic pressure increases also to fish life, especially for embryonic The developmental produce a significant effect. How high the concentration of the solution (or how much of the osmotic pressure) before the life of the fish, especially embryonic development have a significant impact? Related experiments this experiment do to solve this problem. Experimental results show that a lower concentration of glucose, sucrose, can significantly speed up the zebrafish embryonic development speed, and to shorten the incubation time, the embryos incubated and hatched larvae from the solution of the appropriate concentration of sucrose or glucose, in the external shape and motion behavior The other aspects were not found to have abnormalities. 2% sucrose and 1.5% dextrose its hatching time advance significantly shorter larval development in good condition after hatching. However, with a further increase of the concentration of the glucose solution, embryo mortality was significantly higher, even if the individual hatching malformation or death; sucrose solution (8%) of the high concentration of the harmful effects on embryonic development performance is not obvious. 0.25%, 0.5% NaCl can significantly shorten the incubation time of the zebrafish embryos, especially 0.25% NaCl, so incubation time of about 20 hours in advance, and after hatching developmental state. Conclusion: The appropriate concentrations of sucrose and glucose solution can significantly promote the development of the zebrafish embryos; high concentration glucose solution to make most of the embryonic development hindered, a higher concentration of sucrose solution on zebrafish embryonic development without significantly adverse effects. This is most likely due to lower the molar concentration of the sucrose solution of the same weight percentage concentration, caused by the reasons for the smaller the increase in the pressure of infiltration love. Low concentrations of sodium chloride solution can significantly promote zebrafish embryonic development; high concentrations of sodium chloride solution can make the most of the embryonic development blocked, even if the individual hatching many deformities, and all died within 24 hours.