Dissertation
Dissertation > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > General Animal Science > Feed

Study on the Effect of Phytase on Availability of Phytate Phosphorus in Plant Feed

Author FuXueMei
Tutor WangZhiSheng;ZhouAnGuo
School Sichuan Agricultural University
Course Animal Nutrition and Feed Science
Keywords Phytase Rapeseed meal Cottonseed meal Phytate Utilization
CLC S816
Type Master's thesis
Year 2007
Downloads 195
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In this study, two pilot studies of phytase on phytate phosphorus utilization of rapeseed meal and cottonseed meal. Experiment 1, pepsin - trypsin two-step enzymatic hydrolysis in vitro studies of the impact of phytase rapeseed meal, cottonseed meal phosphorus dialysis rate. The results show that: phytase significantly increased rapeseed meal and cottonseed meal phosphorus dialysis rate (P <0.01), and the amount of phytase and phosphorus dialysis rate has a significant dose-response relationship (P <0.01) , When phytase greater than 750U/kg, phytase less degradation of phytate in rapeseed meal and cottonseed meal. Experiment 2, a 3 × 3 Latin square design to select 12 of the average weight of 42 ± 5kg DLY crossbred barrows use balancing test method, the study of phytase on phytate utilization of rapeseed meal, cottonseed meal impact. The test is divided into four groups, the first group to the control group (basal diet group), second, third, four groups were in the first group on the basis to add 250U/kg, 500U/kg, 1000U/kg phytase. Corn - soybean meal diets based diets, rapeseed meal, cottonseed meal, accounts for 20.1% of the total phytate content of the analyte dietary phytate preparation of two under test diets. Each round of the trial period, including 8 days (pre-trial period of six days, the official test period of 2 days), all feces, urine collected in a formal trial period. Determination of phytate and total phosphorus content of excreta. The test results show that: 1. Phytase supplementation significantly increased the digestibility of phytate rapeseed meal, cottonseed meal ((P <0.01), rapeseed meal, cottonseed meal, the amount of phytate phosphorus digestibility and phytase were highly significant quadratic relationship ((P <0.001). phytase amount were when 945U/kg and 873U/kg, and rapeseed meal, cottonseed meal phytate digestibility optimal 86.27%, respectively and 83.35%. apparent digestibility of phytate digestibility and total phosphorus There was a significant linear relationship (P <0.001). rapeseed meal, cottonseed meal, total phosphorus digestibility and deposition rate with phytase amount the increase of, add 250U/kg phytase, 36.8% and 27.7% of the total phosphorus apparent digestibility of rapeseed meal and cottonseed meal increased total phosphorus deposition rate of 23.7% and 32.3%; Add 500U/kg sik acid enzymes, significantly improve the apparent digestibility of rapeseed meal and cottonseed meal total phosphorus 56.5% and 45.2% (P <0.05); improve the total phosphorus deposition rate of 64.2% and 53.0%; When phytase dosage 1000U / kg, extremely significantly improve the apparent digestibility of rapeseed meal and cottonseed meal total phosphorus 95.8% and 88.8% (P <0.01); significantly increased the total phosphorus deposition rate of 100.5% and 85.4% (P <0.01). 3 phytase to reduce phosphorus excretion of rapeseed meal and cottonseed meal, rapeseed meal and cottonseed meal phosphorus When the phytase dosage 1000U/kg, the excretion were significantly reduced by 28.5% and 24.4% (P <0.05 ) sum, phytase can significantly improve the use efficiency of rapeseed meal and cottonseed meal in phytate. suitable phytase in rapeseed meal and cottonseed meal additive the respectively 945U/kg and 873U / kg with the increase in the amount of phytase, rapeseed meal and cottonseed meal total phosphorus digestibility and deposition rate increases, and its total phosphorus digestibility of phytate digestibility highly significant linear relationship.

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