Isolation, Identification and Sensitive Drugs on Mycoplasma Ovipneumoniae
|School||Sichuan Agricultural University|
|Course||Clinical Veterinary Medicine|
|Keywords||Mycoplasma Sheep Mycoplasma pneumoniae Isolation and identification of Drug trials|
Sheep mycoplasma pneumonia by the sheep of Mycoplasma pneumoniae (the Mycoplasma ovipneumoniae, MO, referred to as Y 98 ) a respiratory disease caused by goats and sheep are the most common. In recent years, the disease is prevalent in many countries and regions in the world, the sheep industry caused huge economic losses, harm and serious. However, due to the physiological characteristics of sheep Mycoplasma pneumoniae, cultured in vitro, resulting in few reports of research in this area. This experiment was conducted by Isolation and identification of Ya'an sheep Mycoplasma pneumoniae and in vitro drug testing and other aspects of research approaches and provide scientific basis for prevention and treatment of disease for livestock production and veterinary clinic. From the the Ya'an surrounding area suspected disease disease goat acquisition sample isolates, manager, characteristics and serological identification, confirmed isolates with sheep Mycoplasma pneumoniae standard strain Y98 for the same pathogens, 37 ° C, pH 7.8 medium than the Good growth, and the best growing medium with 20% horse serum; substantially no growth was 5% and 10% horse serum medium or poor growth. In the initial isolation and culture, it is best to use a 37 ° C anaerobic culture anaerobic culture colonies time shorter than 37 ° C aerobic incubation time, and the more typical form. Isolates colonies without a central umbilical papillary. \Ultrathin sections of electron microscopy observation of the bacterial cell wall, cell membrane by the three-unit membrane, intracellular contains granular ribosomes outside the membrane layer of fine hair, darker colored, like the layer of capsular, spherical its basic form; mycoplasma was observed by negative staining pleomorphic, spherical, rod-shaped, spiral and irregular morphology, size 0.2 to 0.4um. Animals regression tests showed that isolates Lamb highly pathogenic, especially for relatively pure lines lamb, there are pathogenic for adult goats, but lower morbidity. Twice by tube dilution method florfenicol, ciprofloxacin, tilmicosin, neomycin, azithromycin, erythromycin, enrofloxacin, levofloxacin, tylosin, difluoro gatifloxacin, doxycycline, tiamulin 12 antimicrobial drugs isolates in vitro minimum inhibitory concentration (MIC) were 0.1008 ± 0.0349,0.0385 ± 0.0167,0.0416 ± 0.0180,6.6612 ± 2.3050,0.0417 ± 0.018,1.2667 ± 0.4388 , 0.2054 ± 0.0712,0.3646 ± 0.1263,0.1917 ± 0.0664,3.2567 ± 1.1281,0.7415 ± 0.2569,0.0417 ± 0.0144. The tests showed that ciprofloxacin, azithromycin and tiamulin four drugs are effective drugs for the treatment of sheep infected with Mycoplasma pneumoniae tilmicosin. This is the joint drug testing and further development of the sheep Mycoplasma pneumoniae efficient, inexpensive compound new veterinary laid the foundation.