Study on Genetic Variation and Interspecific Relationship of Zoysia Willd.
|School||Nanjing Agricultural College|
|Course||Ornamental Plants and Horticulture|
|Keywords||Zoysia Interspecific relationship Genetic variation External traits mark Allozyme markers SSR molecular markers|
Zoysia (Zoysia Willd.) Turfgrass has a very low demand for fertilizers, resistant to drought, heat, salt, cold, etc., and slow growth, and does not require regular pruning, is turfgrass good. Zoysia varieties and development is far from meeting the needs of the market, currently used in Zoysia plant varieties developed by genetic base is very narrow, the discovery and use of genetic resources is the key to obtaining a breeding breakthrough. Zoysia in not as turfgrass developed several kinds have a very good trait and is not being used to sort out the genetic relationship between Zoysia species to explore more genetic resources for guidance hybrid breeders cultivate new varieties of services. This is the first use of morphological markers, allelic enzyme markers, SSR molecular markers on three different levels of genetic marker technology combined with the geographical distribution and interspecific hybridization test Zoysia 9 1 variant 59 provenances analysis of genetic variation, comparing the size of intraspecific genetic differentiation and phylogenetic relationship of these species in the evolutionary process. The main conclusions are as follows: 1, through observation and analysis of Zoysia 23 external traits, concluded: ① ditch Ye junction strand (Z. matrella (L.) Merr) intraspecific variety of sources in many traits on there are a lot of variability zoysiagrass (Z.japonica Steud) and the Chinese Zoysia japonica (Z.sinica Hance), intraspecific variation, followed Zoysia matrella, and both species variation within size closer to. North Korea zoysiagrass (Z. koreana Mez) and Zoysia tenuifolia (Z. tenuifolia Thiele) between the respective provenance of many external traits than similar intraspecific variation; (2) by using cluster analysis found that these species according Genetic distance can be divided into three categories: the first category Zoysia, Zoysia China, North Korea of ??Zoysia first poly together after the big spike zoysiagrass (Z. macrostachya Franchet et Sav) together into a large class of ; second category the ditch Manilagrass, and Zoysia tenuifolia first clustered into the subcategories, the long flower Zoysia (Z.sinica var. nipponica Ohwi) and small Zoysia (Z.minimaZotov) clustered into another subclasses, then these species clustered into categories. Large flower Zoysia (Z.macranthaDesvaux) alone self-contained cluster for the third class; ③ The principal component analysis results show that: blade width, stolon length, diameter, of spikelets per spike, PEDICEL length, spikelet length, reproductive branches height, the ligule colors and with or without hair 9 traits of Zoysia study of the genetic relationship between plant species has important significance. Malate dehydrogenase (MDH) analysis of Zoysia material esterase (EST) and peroxidase (POD) three allozyme characteristics, the genetic relationship between their species, the results show that: ① within genetic differentiation order: the Zoysia matrella gt; the Zoysia gt; Zoysia sinica gt; North Korea Zoysia; ② clustering results show: Zoysia, Hance, long flower Zoysia , Zoysia macrostachys poly first class; Zoysia matrella Zoysia tenuifolia first clustered into one group, and then a small Zoysia Zoysia Pacific (Z.pacifica Hotta), large flower Zoysia gathered in this class; North Korea Zoysia category of their own. 3, SSR molecular marker genetic relationship analysis of Zoysia material, the results showed that: ① The intraspecific genetic differentiation order: to the Zoysia, China Zoysia gt; North Korea Zoysia gt; ditch leaf junction strand grass; ② clustering results show: Zoysia, Zoysia China, North Korea Zoysia and big spike Zoysia clustered into one class; of Zoysia matrella small Zoysia japonica and Zoysia tenuifolia three kinds a class; big flower Zoysia The long flower Zoysia each as a class. Binding location analysis and interspecific hybridization test, according to three genetic markers derived interspecific relationships Zoysia 9 kinds of variants: Zoysia and China Zoysia most near dendrogram, phylogenetic relations recently. The genetic distance between the big spike Zoysia japonica and Zoysia japonica and Zoysia sinica. Zoysia matrella and Zoysia tenuifolia close together on the dendrogram small Zoysia closer to the two kinds of genetic distance. The genetic relationship between the large flower Zoysia and other grass far. Korean Zoysia according to the results obtained by the the external traits markers and SSR markers: very close genetic relationship between Zoysia Zoysia sinica, but the geographic distribution and species hybridization, although the results are inconsistent with allozyme supported by the data. The long flower Manilagrass clustering results obtained in the three labeled in a different location. Pacific Zoysia allozyme markers based on results with the Zoysia matrella Zoysia tenuifolia and small zoysiagrass poly. Pacific Zoysia not involved in clustering analysis in the other two markers. 5, according to the three genetic markers analysis Zoysia is a genetic variation in the size of the plant species: the Zoysia, China Zoysia gt; North Korea Zoysia. And Zoysia japonica and Zoysia sinica two kinds of the degree of intraspecific variation is very close. The ditch Manilagrass based on external traits and allozyme markers for intraspecific variation is very large, its variation is small and based on the results of SSR molecular markers.