Studies on the Chemical Constituents of Patrinia Heterophylla Bunge. and Their Anti-tumor Activities
|School||Northwest Normal University|
|Keywords||Different Patrinia Chemical composition Triterpenoid Cytotoxicity Hederagenin Apoptosis|
This thesis, under the guidance of in vitro cytotoxicity detection SRB assay different Patrinia the methanol extract of petroleum ether, chloroform, ethyl acetate and n-butanol extract were the ingredients of anti-tumor activity tracking Isolation and Screening. Experimental results show that the chloroform extract on human hepatoma cell line HepG2 human gastric carcinoma cell line SGC-7901 and human promyelocytic leukemia cell line HL-60 cells are the most toxic, IC 50 , respectively : 19.57 ± 2.54μg/mL, 44.04 ± 5.04μg/mL and 17.91 ± 0.88μg/mL; petroleum ether, chloroform, ethyl acetate and n-butanol extract of human promyelocytic leukemia cell line HL-60 The cytotoxic effect most obvious the the IC 50 , respectively: of 33.72 ± 5.8μg/mL, the 17.91 ± 0.88μg/mL, 31.49 from ± 3.70μg/mL, and 40.23 ± 7.39μg ╱ mL of. Visible chloroform extract was the most toxic of human promyelocytic leukemia cell line HL-60 cells. For further object of study, were isolated by column chromatography on silica gel to give 18 of the monomer compound to the cells of the most toxic chloroform extracts. The use of modern spectroscopic methods (NMR, MS, IR, UV) identified 11 compounds, compounds 1 to 11 were: cork triterpenoid ketone, beta-sitosterol, oleanolic acid, ursolic acid, Hederagenin the daucosterol, crabapple fruit alcohol, 7 - hydroxy-6 - methoxycoumarin oleanolic acid-3-O-α-L-pyran arabinoside β-Hong Overruns and α-Hong Overruns ; seven triterpenoids, two steroids, triterpenoid saponins and coumarins compounds each one. Except oleanolic acid, the remaining compounds from this plant for the first time isolated triterpenoid ketone cork, the Malus alcohol, β-Hong Overruns and α-Hong dryad four compounds for the first time from the genus were isolated. The SRB assay cell toxicity testing, the results show that the higher yield compounds oleanolic acid, ursolic Hederagenin 7 - hydroxy-6 - methoxy coumarin and oleanolic acid - 3-O-α-L-pyran arabinoside on human hepatoma cell lines HepG2, human gastric carcinoma cell line SGC-7901 and human promyelocytic leukemia cell line HL-60 has strong cytotoxicity, IC 50 of 4.56 ± 0.48μg/mL ~ 18.07 ± 0.05μg/mL. Also found that the the isolated triterpenoid Compound C-28 carboxyl group (-COOH) is its cytotoxic effect of the reactive groups. A preliminary study further of triterpenoids Hederagenin vitro antitumor mechanisms hope staining blue row by inverted microscope and Taiwan, found Hederagenin can significantly inhibit the growth of human promyelocytic leukemia cell line HL-60 ; and its lethal mechanism through Hoechst33258 staining and DNA ladder electrophoresis studies found Hederagenin can induce human promyelocytic leukemia cell line HL-60 apoptosis.