The Extraction and Purification of Luteolin from Peanut Shell
|Keywords||Peanut shells Luteolin Extract Separate|
Peanut shells, in addition to containing carbohydrate and crude fiber material also contains a variety of flavonoids. Luteolin is one of the main flavonoids, anti-bacterial and anti-inflammatory, antispasmodic, expectorant, anti-oxidation, anti-tumor and other pharmacological activities. Peanut shell is an inexpensive and renewable natural resources, such as extraction and separation of the active ingredient luteolin, the product was used for drug or health food production, and has broad market prospect. In this paper, enzyme pretreatment - alcohol extract combination process of the enzymatic hydrolysis pH value, reaction temperature, reaction time and enzyme dosage of peanut shells flavonoids luteolin extraction. Experimental results show that the the optimal enzyme pre-extraction conditions were as follows: pH 5.4, hydrolysis temperature 50 ° C, hydrolysis time 1.5 h, 0.10% of the amount of enzyme. In the optimal pretreatment conditions, luteolin extraction yield reached 2.83 mg · g -1 sup>. Meanwhile, based on Fick's second law, the alcohol solution extraction process established the extraction kinetics model. The extraction yield of luteolin experimentally measured data with the extraction time change, calculate the rate constant (k), the activation energy (Ea) and the effective diffusion coefficient (D) kinetic parameter values, extract luteolin process design and optimization of operating conditions to provide a theoretical basis. Column chromatography purification technique to study the thermodynamic and kinetic characteristics of the polyamide resin static adsorption luteolin in peanut shells. Experimentally measured adsorption temperature line in line with the Langmuir adsorption temperature and type and Thermodynamic Functions calculated to obtain the adsorption enthalpy (△ H), free energy (ΔG) and adsorption entropy (ΔS), results showed that the adsorption process is spontaneous conduct and accompanied put thermal physical adsorption process. Also proposed a reaction and pseudo-second-response model describes the adsorption kinetics data, concluded that the proposed two adsorption kinetics model to better describe the adsorption behavior of polyamide resin luteolin. In addition, the breakthrough curves were measured by dynamic adsorption experiments, polyamide resin luteolin dynamic adsorption capacity (Q) of 0.2817 mg the · g -1 sup>, the overall mass transfer coefficient (KFav) 0.0570 s -1 sup>, mass transfer length (ZA) 28.40 cm enlargement operation of luteolin production provides an experimental and theoretical basis. Luteolin in the extract of peanut shells, identified by the best results of 75% ethanol solution eluted by different proportions of alcohol solution gradient elution. Molecular imprinting technique, luteolin as the template molecule, alpha-methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethyl acrylate (EDMA) as crosslinker, azobisisobutyronitrile (AIBN ) as initiator, acetone as porogen by bulk polymerization, Luteolin molecularly imprinted polymer (MIP) was prepared. By static adsorption experiments, the selectivity and adsorption properties of the polymer luteolin. The results showed that the imprinted polymer for the template molecule a special imprinting effect, luteolin has the obvious selectivity and the ability to identify and blank polymer (NIP). Loaded by the molecularly imprinted polymer solid phase extraction column to determine the first with 55% methanol solution as eluent cleaning, and then methanol eluted, to the greatest degree of separation of mixed flavonoid luteolin - apigenin compound luteolin. At the same time molecularly imprinted polymer applied to the separation of the peanut shell extract luteolin, in leaching and elution conditions, selective separation of the target product luteolin, its purity by 2.51% to 93.40 % and recovery of 92.84%.