The Effect of Embedding Compound Including Auto-bone Marrow Mesenchymal Stem Cells and Calcium-algitate on Denervated Skeletal Muscle Atrophy in Rabbits
|Course||Human Anatomy,Histology and Embryology|
|Keywords||Bone marrow mesenchymal stem cells Muscle Atrophy transplant basic fibroblast growth factor|
Objective: Denervated skeletal muscle atrophy is one of the critical complications of the lateral peripheral nerve injuries. Nowadays, how to delay and relieve muscle atrophy is a tough problem in peripheral nerve territory. Establishing rabbits models of denervated gastrocnemius, we transplant auto-bone marrow mesenchymal stem cells and calcium-algitate into the denervated Gastroc. Consequently, we add the bFGF and streptomycin (ST) in the micro-circumstances when embedding the carrier compound in order to improve the effect of the transplantation.method:Bone marrow is obtained from the iliac bone of 20 rabbits respectively. As the seed cells, Mesenchymal Stem Cels are purified with the density gradient centrifugation and ampilifid. The cell suspension is prepared with the 3th generation and bFGF/ST, then finishing the carrier compound and embedding. The animals were divided randomly into four groups. Animal models of denervated gastrocnemius of calf were set up after tibial nerves were transected. Group(A)were embedded with carier compound as the experimental, Group(B)were embedded without bFGF/ST, implantation of calcium alginate exclusively as group(C), group(D)remains blank as control.On postoperative 30d and 60d, electromyogram (amplitude of fibrillation potential), wet muscle weight,myocyte diameter and cross sectional area were checked. We analysised the initial date by SAS 6.12 software package of statistics, P<0.05 hints the significant differences.Results: During the early 2 weeks, skin threptic deficiency was found in experiment toe of all groups. On 30 days after operation, sural muscle atrophy appeared. On 30 days after operation, sural muscle of calf wet weight was 6.68±0.12 g in the Group A, 5.41±0.10 g in the Group B, 4.21±0.06 g in the Group C and 4.18±0.10 g in the Group D. With no significant difference among groups A and B (P>0.05), there was significant difference between groups A, B and C, D respectively. On 60 days,wet weight was 5.42±0.08 g in the Group A, 5.17±0.05 g in the Group B, there was significant difference between groups A and B(P<0.01) .3.10±0.07 g and 3.10±0.07g for Group C and Group D. there was no significant difference between Group C and Group D (P>0.05). On 30days postoperatively, myocyte diameter was 25.43±0.76um for Group A and 20.32±0.42 um for Group B, there was significant difference between group A and B(P<0.05). there was no significant difference between group D (13.52±0.14um) and Group C(12.96±0.12um) (P<0.05). on 60days,myocyte diameter was 20.12±0.54um in the Group A and 16.57±0.42 in the Group B, there was significant difference between group A and B(P<0.01). 11.86±0.37um in the Group D, 11.78±0.58um in the Group C. Results between group c and group D had no significant difference (P>0.05). The difference between A, B groups and C, D groups has statistically significant. 30 and 60days postoperatively, Results of myocyte cross sectional area between group C and group D had no significant difference (P>0.05). groups A and B were increased compared with group C and group D (P<0.05). The differences between groups A and B has statistically significant (P<0.05). The phenomena in the MALLORY staining: hyperplasia of collagen fiber can be found among muscle slice of all groups. The amplitude of fibrillation potential could be observed in all Groups. On 30days and 60days, the amplitude of the Group A was higher than group B (P<0.05), groups A, B were higher than groups C, D respectively. Results between group C and group D had no significant difference (P>0.05 ).Conclusion: implantation of auto-MSCs and calcium alginate may relieve denervated Skeletal Muscle Atrophy in rabbit. Adding bFGF and ST has positive function to delay and relieve muscle atrophy when embedding the compound composed of auto-MSCs and calcium Alginate.