Dissertation > Agricultural Sciences > Gardening > Vegetable gardening > Solanaceous > Chili

Analysis of Genetic and Molecular Markers of Fertility Restoration Major Gene of Cytoplasmic Male Sterility in Pepper (Capsicum Annuum L.)

Author AiLiang
Tutor ZhuQiZhong;ZhangBaoZuo
School Shandong University
Course Biochemistry and Molecular Biology
Keywords Chili Cytoplasmic male sterility Fertility restoration Molecular markers Homology comparison
CLC S641.3
Type Master's thesis
Year 2011
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Pepper (Capsicum annuum L.) is an important vegetable crops. Pepper significant heterosis, hybrid varieties in the production of a wide range of applications. Hybrid Pepper seed production mainly rely on manual emasculation and artificial pollination, the application of pepper cytoplasmic male sterility supporting the Department of seed can reduce labor costs, intellectual property protection, and can improve the purity of the seed. Application of three lines supporting breeding cytoplasmic male sterility restore the Line of sterile difficult problem. Find pepper fertility restorer gene Rf (fertility restorer) closely linked markers, you can further leverage chromosome walking was cloned Rf genes to transgenic turn to fertility restorer line. This article pepper cytoplasmic male sterility recovery of genetic analysis, screening a large number of molecular markers and comparative genomic approach to developing markers. 1. 2862 segregating population 77013A × (Yolo Wonder × Perennial) to plant fertility investigate. The survey results show that the the fertile plant infertility monoclonal proportion not fully consistent with the proportion of fertility restoration is controlled by a single gene genetic. Environmental factors on the fertility of cytoplasmic male sterility in recovery has a certain impact. (2) screened a total of 1,366 pairs of primers, one pair of SCAR markers, five pairs of CAPS markers, 417 pairs of SSR markers, 495 pairs of SRAP markers and 448 pairs of AFLP markers. Find a gene SCAR marker linked to the same recovery. Verification of the separate groups in 2862 SACR-CRF mark 1419 performance with dominant bands (approximately 500 bp) with Rf gene linkage, 1443 with unconditional the same rf genetic linkage, is not clear and can not be determined 2, x2 = 0.236, P = 0.627, consistent with the expected ratio of 1:1. After 200 fertility survey results the analysis and Joinmap4.0 software calculated SCAR markers restorer gene Rf chain distance 4.3cM. Verified in 35 were the SACR-CRF marked exactly the same molecular markers show results and material recovery phenotype. The other Rf genes have been cloned and pepper has been published EST and tomato genome sequencing, pepper 6 the high similarity EST sequence, six EST sequences designed 43 pairs of primers filter. In accordance with the high similarity section to select the mark on the tomato chromosome T6 for screening. The will the SCAR-CRF mark specific amplified fragments were sequenced, the fragment length of 556 bp compared to the tomato genome, approximately 100bp fragment with Tomato T6 chromosome fragment homologous sequence similarity of 86%. Homologous gene sequence and recovery to be further studied.

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