Dissertation > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical

Chemical Constituents from Asterina Pectinifera and Pentaceraster Chinensis and Their Biological Activities

Author PengYan
Tutor ZhengJianXian
School South China University of Technology
Course Of Food Science
Keywords Starfish Chemical composition Swallow China warts starfish Biological activity
CLC R284
Type PhD thesis
Year 2010
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In recent years, with the development of aquaculture in China and changes in the environment, the growth and reproduction of the starfish to create the conditions, starfish flood event brought serious losses to the shellfish farmers, therefore, the starfish is considered to be a species detrimental to the animals, most of the starfish were discarded. In this paper, the rich resources in the Yellow Sea Swallow and the South China Sea and China warts starfish to development of starfish biological resources as raw materials, the first basic nutrients, saponins and anti-Artemia activity to evaluate its nutritional and medicinal value, and then using silica gel column chromatography, macroporous resin adsorption column chromatography, Sephadex LH-20 size exclusion chromatography, preparative thin layer chromatography and HPLC chemical composition of the two starfish ethanol extract were isolated and purified by NMR, IR, MS, chemical degradation and literature monomer compounds of structural identification than on a variety of methods, followed by some of the compounds, anti-cancer, anti-viral and anti-bacterial activity test, and analysis of the activity and structure of the compound, the main results are as follows: 1, petrels contains more high protein (29.37%) and fat (10.18%), with a higher nutritional value, the body also contains saponins (48 mg/100g), 24 hours of ethanol extracts of Artemia larvae LC50 for 587.8μg/ml Swallow has some medicinal value; China warts starfish also has a high nutritional value, the body contains high protein (12.08%) and low fat (1.66%), and also contains saponins (105 mg/100g), ethanol extracts of Artemia larvae 24 hours LC50 was 124.1μg/ml, and China warts starfish also has some medicinal value. 2 from Swallow vivo isolated 40 compounds were identified of which 29 (A1 29), including six nucleotides, 7 sterol, a sterol sulfate, more than one hydroxy-sterol fatty acid ester, 2 saponins, a ring with a dipeptide, a stigmasterol glycoside and 10 other types of compounds. Compound A13 is a new compound, compound A3, A5, A11, A17, A18 and A23 A 28 for the first time isolated from Swallow body. 3, using the MTT method, CPE method and disc agar diffusion method on the part of the compounds isolated from Petrel vivo activity test, and the results showed that compound A11, A16 and A18 of HepG2 cells showed a different toxic toxic concentration of 50 1 00μg/ml, A11, A15, A16, A18, A19 and A21 in 50 1 00μg/ml of HSV-1 cytopathic effect showed varying degrees of inhibitory activity A3, A10, A12, A13, A20, A22, A24 and A29 showed a significant inhibitory activity on the growth of Escherichia coli or Staphylococcus aureus. 4, obtained from China warts starfish vivo isolated 22 of the monomer compound were identified, of which 13 (the PC1, P C13), including 4 ring dipeptide, two sterol sulfate, an ergosterol an indole acid 2 glycerides glycoside and three other types of compounds. Which compound PC1 P C12 for the first time from the separation of the species. Vivo separation from China warts starfish get some of these compounds in vitro anticancer, antiviral and antibacterial activity test results showed that the compounds PC7 and PC8 pair HepG 2 cell toxicity, toxic concentration of 50 1 of 00μg/ml, PC1, PC2, PC3, PC4, PC5, PC6, PC7, PC8, PC9 and PC10 growth of Escherichia coli or Staphylococcus aureus showed significant inhibitory activity. 6, through the relationship between the activity of the compound and structure of the comparative analysis, found that the compound of HepG2 display cytotoxic activity may be glycine structure, the sulfate on the steroidal nucleus, 15β-OH, on the sterol side chain of the 24 - OH and △ 22 (23) double bond group related inhibition of HSV-1 activity may be associated with the 15-OH and sulfate on the steroidal nucleus, steroidal side chain on the C-24 position of arabinose, 24 - OH and △ 22 (23) double bond, etc. groups, antibacterial activity may be donor or acceptor, the hydrogen bonds in the structure of the ribose, a hydroxyl group, NH, ring dipeptide sulfate, a steroidal nucleus, the double bonds , galactose, glycerol, and proline, etc. related to the group.

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