Preparation of Silk Fibroin Peptide/ Polyethylene Glycol Compound and Study of Its Biocompatibility
|School||Chongqing University of Technology|
|Keywords||Nanowire fibroin Polyethylene glycol Graft Biocompatibility|
Silk fibroin (Silk Fibroin, SF), a natural protein fiber , is one of the proteins of the human use of a very early . In recent years , numerous the study table Mingsi fibroin has good biocompatibility characteristics of the extremely slow degradation rate , low immunogenicity , a performance of biological materials . Silk fibroin , there are a variety of defects in the application process often regenerated silk fibroin (SF) modified , modified and become hot . Through polymer reaction using polyethylene glycol modified silk fibroin nanoscale , the development of a new type of biological material , and a preliminary study of its biological properties . First, extracted from the cocoon of pure silk fibroin , then digested by high concentrations of dissolved inorganic salts prepared nano- silk fibroin peptide ( SFP ) ; and then acylated polyethylene glycol , by a polymer reaction grafted onto the nanowire fibroin peptide molecules obtained by nanoscale silk fibroin peptide / polyethylene glycol composite compounds , the tests showed that the optimal feed ratio nanowire fibroin peptide with polyethylene glycol acylate 1 : 10 (mass volume ratio) , yield 93.8% (weight ratio) can be achieved . Silk fibroin peptide / polyethylene glycol compound of the nanoscale characterized using infrared spectroscopy , amino acid analysis , and amino acid analysis showed that the enzymatic not destroy the activity of the reaction zone of the silk fibroin . Infrared spectral characterization confirmed successfully grafted onto the polyethylene glycol nanowire fibroin peptide molecules . Nanoscale silk fibroin peptide / polyethylene glycol compounds , a performance test , wherein the solubility test showed that after the reaction the silk fibroin peptide enhanced stability , will not dissolve in the calcium chloride in the neutral salt solution . The in vitro cytotoxicity shows the preparation of the composite obtained for L-929 mouse fibroblast cells toxicity was evaluated as a 0 or a 1 , no cell toxicity , and also shows good promoting cell growth performance . Antibacterial experimental results show that the nanowire fibroin peptide before and after the reaction , E. coli and Staphylococcus aureus antibacterial effect . Therefore , nanowire FIBROIN / polyethylene glycol compound used as a biological material or a pharmaceutically acceptable polymer material application feasibility .